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1 Department of Molecular and Structural Biology, Aarhus University, Gustav Wieds Vej 10C, DK-8000 Aarhus C, Denmark
2 Department of Clinical Microbiology, Aalborg Hospital, Aalborg, Denmark
Author for correspondence: Hans U. Sperling-Petersen. Tel: +45 8942 5050. Fax: +45 8618 2812. e-mail: husp{at}biobase.dk
ABSTRACT
Nucleotide sequence analysis is increasingly being used to identify bacteria. In this work, a PCR assay based on degenerate primers was used to obtain the partial sequence of infB, the gene encoding translation initiation factor 2 (IF2), in 39 clinical isolates of different Enterobacteriaceae. The partial sequence encodes the GTP-binding domain of IF2. Together with sequences from the literature, a total of 15 species, each represented by one to seven strains, was investigated. Phylogenetic analysis yielded an evolutionary tree which had a topology similar to a tree constructed using available 16S rRNA sequences. It is concluded that the inter-species variation of the infB gene fragment is sufficient for its use in the characterization of strains that have aberrant phenotypic reactions.
Key Words: infB • translation initiation factor 2 • phylogenetic analysis • Enterobacteriaceae
Present address: IBMC, Universidade do Porto, Rua do Campo Alegre, 823, 4150 Porto, Portugal.
The EMBL accession numbers for the sequences in this paper are given in Table 1.
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