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Int J Syst Bacteriol 49 (1999), 1493-1511; DOI 10.1099/00207713-49-4-1493
© 1999 Society for General Microbiology
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Mycobacterium wolinskyi sp. nov. and Mycobacterium goodii sp. nov., two new rapidly growing species related to Mycobacterium smegmatis and associated with human wound infections: a cooperative study from the International Working Group on Mycobacterial Taxonomy

Barbara A. Brown1, Burkhard Springer3, Vincent A. Steingrube1, Rebecca W. Wilson2, Gaby E. Pfyffer4, Maria J. Garcia5, M. Carmen Menendez5, Beatriz Rodriguez-Salgado5, Kenneth C. Jost, Jr.6, Sher H. Chiu6, Grace O. Onyi1, Erik C. Böttger3 and Richard J. Wallace, Jr1

1 Department of Microbiology, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708–3154, USA
2 The Center for Pulmonary and Infectious Disease Control, The University of Texas Health Center at Tyler, 11937 US Hwy 271, Tyler, TX 75708–3154, USA
3 Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, 30625 Hannover, Germany
4 Swiss National Center for Mycobacteria, Department of Medical Microbiology of the University of Zörich, CH-8028 Zürich, Switzerland
5 Departmento de Medicina Preventiva, Facultad de Medicina, Universidad Autonoma de Madrid, Madrid, Spain
6 Texas Department of Health, Austin, TX, USA

Author for correspondence: Barbara A. Brown. Tel: +1 903 877 7682. Fax: +1 903 877 7652. e-mail: babrown{at}uthct.edu

ABSTRACT

Previous investigations demonstrated three taxonomic groups among 22 clinical isolates of Mycobacterium smegmatis. These studies were expanded to 71 clinical isolates, of which 35 (49%) (group 1) were identical to five ATCC reference strains including the type strain ATCC 19420T. Twenty-eight isolates (39%) were group 2, and eight isolates (11%) were group 3. Isolates of groups 2 and 3 were most often associated with post-traumatic or post-surgical wound infections including osteomyelitis, were susceptible to sulfamethoxazole, amikacin, imipenem and the tetracyclines, variably resistan to clarithromycin, and susceptible (group 1), intermediately resistant (group 2) or resistant (group 3) to tobramycin. The three groups were similar by routine biochemical and growth characteristics, but had different mycolic acid dimethoxy-4-coumarinylmethyl ester elution patterns by HPLC and different PCR-restriction enzyme patterns of a 439 bp fragment of the hsp-65 gene. Group 3 isolates differed from group 1 by 18 bp by 16S rRNA sequencing and exhibited < 25% homology by DNA-DNA hybridization, being most closely related to Mycobacterium mageritense. The 16S rRNA of group 1 and group 2 isolates differed by only 3 bp, but by DNA-DNA hybridization they exhibited only 40% homology. The following names are proposed: Mycobacterium goodii sp. nov. for group 2 isolates (type strain ATCC 700504T = M069T), Mycobacterium wolinskyi sp. nov. for group 3 isolates (type strain ATCC 700010T = M0739T) and Mycobacterium smegmatis sensu stricto for group 1 isolates.

Key Words: Mycobacterium goodii • Mycobacterium wolinskyi • Mycobacterium smegmatis • rapidly growing mycobacteria

The EMBL accession numbers for the 16S rRNA sequences in this paper are Y12873 (ATCC 700010T), Y12871 (ATCC 700009) and Y12872 (ATCC 700504T (M069T).




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