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Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis

Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Ap. P. 565-A, Cuernavaca, Morelos, Mexico

Author for correspondence: Esperanza Martínez-Romero. Tel: +52 73 13 16 97. Fax: +52 73 17 55 81. e-mail: emartine{at}cifn.unam.mx

ABSTRACT

Fifty rhizobial isolates from root nodules of Mimosa affinis, a small leguminous plant native to Mexico, were identified as Rhizobium etli on the basis of the results of PCR-RFLP and RFLP analyses of small-subunit rRNA genes, multilocus enzyme electrophoresis and DNA-DNA homology. They are, however, a restricted group of lineages with low genetic diversity within the species. The isolates from M. affinis differed from the R. etli strains that originated from bean plants (Phaseolus vulgaris) in the size and replicator region of the symbiotic plasmid and in symbiotic-plasmid-borne traits such as nifH gene sequence and organization, melanin production and host specificity. A new biovar, bv. mimosae, is proposed within R. etli to encompass Rhizobium isolates obtained from M. affinis. The strains from common bean plants have been designated previously as R. etli bv. phaseoli. Strains of both R. etli biovars could nodulate P. vulgaris, but only those of bv. mimosae could form nitrogen-fixing nodules on Leucaena leucocephala.

Key Words: Rhizobium etli • Mimosa affinis • symbiotic plasmid • genetic diversity

The GenBank accession number for the nifH gene sequence of isolate Mim2 is AF107621.

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