| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
2 Department of Microbiology, National Institute of Health, Seoul, Korea
3 Department of Microbiology, College of Medicine, Hallym University, Chunchon, Korea
Author for correspondence: Toshiyuki Masuzawa. Tel: +81 54 264 5710. Fax: +81 54 264 5715. e-mail: masuzawa{at}u-shizuoka-ken.ac.jp
ABSTRACT
The 16S rRNA sequences of the Korean Borrelia strains 5MT and 9MT, isolated from lxodes nipponensis, showed identities of 99.0-99.1% to that of B. afzelii. The strains were tentatively classified as belonging to the B. afzelii-related group. In this study, Korean isolates, including these strains, were characterized further and compared with recently described new species. These strains generated a RFLP pattern that has not been found previously in RFLP analysis of the 5S-23S rRNA intergenic spacer and the flagellin gene. When phylogenetic trees were constructed, based on the 5S-23S rRNA intergenic spacer, flagellin gene and 16S rRNA sequences, these Korean isolates formed a cluster with the Borrelia strain Am501 isolated from Ixodes columnae in Japan and Borrelia valaisiana strains VS116T and UK isolated from Ixodes ricinus in Europe and were distinguishable from the other species. However, these three groups of strains were divergent from each other in the molecular masses of the putative outer surface protein A (OspA) and in the sequences of the ospA gene. These findings suggest that these Korean isolates and one Japanese isolate are members of B. valaisiana and that OspA of this species is divergent, as is that of Borrelia garinii. This led to the speculation that B. valaisiana strains are adapted to the vector ticks found in each locality.
Key Words: Borrelia valaisiana • Lyme disease • Ixodes nipponensis • 16S rRNA • Korea
Present address: Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, 625 Pedro Gil Street, Ermita, Manila 1000, The Philippines.
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, 55–235 intergenic spacer, fla and ospA sequences reported in this paper are AB013913–AB013917, AB014677-AB014679, AB016974–AB016979 and AB017479.
This article has been cited by other articles:
|
|
 |
|
  C.-Y. Chu, W. Liu, B.-G. Jiang, D.-M. Wang, W.-J. Jiang, Q.-M. Zhao, P.-H. Zhang, Z.-X. Wang, G.-P. Tang, H. Yang, et al. Novel Genospecies of Borrelia burgdorferi Sensu Lato from Rodents and Ticks in Southwestern China J. Clin. Microbiol., September 1, 2008; 46(9): 3130 - 3133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-Y. Chu, B.-G. Jiang, W. Liu, Q.-M. Zhao, X.-M. Wu, P.-H. Zhang, L. Zhan, H. Yang, and W.-C. Cao Presence of pathogenic Borrelia burgdorferi sensu lato in ticks and rodents in Zhejiang, south-east China J. Med. Microbiol., August 1, 2008; 57(8): 980 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Saito, T. Ito, N. Asashima, M. Ohno, R. Nagai, H. Fujita, N. Koizumi, A. Takano, H. Watanabe, and H. Kawabata Borrelia valaisiana Infection in a Japanese Man Associated with Traveling to Foreign Countries Am J Trop Med Hyg, December 1, 2007; 77(6): 1124 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Anderson and D. E. Norris Genetic Diversity of Borrelia burgdorferi Sensu Stricto in Peromyscus leucopus, the Primary Reservoir of Lyme Disease in a Region of Endemicity in Southern Maryland Appl. Envir. Microbiol., August 1, 2006; 72(8): 5331 - 5341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Younsi, M. Sarih, F. Jouda, E. Godfroid, L. Gern, A. Bouattour, G. Baranton, and D. Postic Characterization of Borrelia lusitaniae Isolates Collected in Tunisia and Morocco J. Clin. Microbiol., April 1, 2005; 43(4): 1587 - 1593. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Masuzawa, N. Hashimoto, M. Kudeken, T. Kadosaka, M. Nakamura, H. Kawabata, N. Koizumi, and Y. Imai New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan J. Med. Microbiol., May 1, 2004; 53(5): 421 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Guner, N. Hashimoto, N. Takada, K. Kaneda, Y. Imai, and T. Masuzawa First isolation and characterization of Borrelia burgdorferi sensu lato strains from Ixodes ricinus ticks in Turkey J. Med. Microbiol., September 1, 2003; 52(9): 807 - 813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Derdakova, L. Beati, B. Pet'ko, M. Stanko, and D. Fish Genetic Variability within Borrelia burgdorferi Sensu Lato Genospecies Established by PCR-Single-Strand Conformation Polymorphism Analysis of the rrfA-rrlB Intergenic Spacer in Ixodes ricinus Ticks from the Czech Republic Appl. Envir. Microbiol., January 1, 2003; 69(1): 509 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. DEMIRKAN, S.D. CARTER, C. WINSTANLEY, K.D. BRUCE, N.M. McNAIR, M. WOODSIDE, and C.A. HART Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot J. Med. Microbiol., December 1, 2001; 50(12): 1061 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ishiguro, N. Takada, T. Masuzawa, and T. Fukui Prevalence of Lyme Disease Borrelia spp. in Ticks from Migratory Birds on the Japanese Mainland Appl. Envir. Microbiol., March 1, 2000; 66(3): 982 - 986. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
