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Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov., isolated from leguminous trees in Sudan and Kenya

Giselle Nick¹, Philippe de Lajudie^2,5,, Bertrand D. Eardly³, Sini Suomalainen⁴, Lars Paulin⁴, Xiaoping Zhang^1,6, Monique Gillis⁵ and Kristina Lindström¹

¹ Department of Applied Chemistry and Microbiology, PO Box 56, Viikki Biocentre, FIN-00014 University of Helsinki, Finland
² Laboratoire de Microbiologic des Sols, ORSTOM BP 1386, Dakar, Senegal
³ Pennsylvania State University, Berks Campus, POB 7009, Reading, PA 19610-6009, USA
⁴ Institute of Biotechnology, POB 56, Biocentre 1, FIN-00014 University of Helsinki, Finland
⁵ Laboratorium voor Microbiologic, Universiteit Gent, K. L. Ledeganckstraat, 35, B-9000 Gent, Belgium
⁶ Department of Applied Microbiology, Sichuan Agricultural University, Yaan 625014, People’s Republic of China

Author for correspondence: Giselle Nick Tel: + 358 9 708 59281, Fax: + 358 9 708 59322. e-mail giselle.nick-maenpaa{at}helsinki.fi

ABSTRACT

SDS-PAGE of total bacterial proteins was applied to the classification of 25 Sudanese and five Kenyan strains isolated from the root nodules of Acacia senegal and Prosopis chilensis. Twenty strains were also studied by multilocus enzyme electrophoresis (MLEE) and the whole 16S rRNA gene was sequenced from two strains representing the two major clusters. These results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, DNA-DNA dot-blot hybridization, genomic fingerprinting using repetitive sequence-based PCR, DNA base composition analysis, DNA-DNA reassociation analysis, partial sequencing of the 16S rRNA gene and RFLP analysis of the amplified 16S rRNA gene, showed that all 30 strains belong to the genus Sinorhizobium. Two of the strains grouped with Sinorhizobium saheli and seven with Sinorhizobium terangae, while the rest did not cluster with any of the established species. The majority of the strains formed two phenotypically and genotypically distinct groups and we therefore propose that these strains should be classified as two new species, Sinorhizobium arboris sp. nov. and Sinorhizobium kostiense sp. nov.

Key Words: MLEE • SDS-PAGE • 16S rDNA sequencing • Sinorhizobium arboris • Sinorhizoöium kostiense

The EMBL accession numbers for the 16S rRNA sequences of S. arboris HAMBI 1552^T and S. kostiense HAMBI 1489^T are Z78204 and Z78203.

Present address: LSTM-ORSTOM/CIRAD-Forêt, Baillarget, BP 5035, 34032 Montpellier, France.

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