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Microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory

¹ Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Minamisawa, Minami-ku, Sapporo 005-8601, Japan
² Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
³ Research Laboratory, Higeta Shoyu Co., Ltd, Choshi, Chiba 288-8680, Japan

Author for correspondence: Hidetoshi Matsuyama. Tel: +81 11 5715111. Fax: +81 11 5717879. e-mail: matsuyama{at}db.htokai.ac.jp

ABSTRACT

Two strictly aerobic, heterotrophic and mesophilic new organisms, strains Kitami A1 and Kitami C2^T, were isolated from the wastewater of a sugar-beet factory in Kitami City, Hokkaido, Japan. In batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (EPSs) utilizing sucrose as the sole carbon source. The G+C contents of the strains Kitami C2^T and Kitami A1 were 69·2 mol%. Both strains had anteiso-C15:0 acid, anteiso-C17:0 acid and iso-C16:0 as major components. The major isoprenoid quinones from these strains included menaquinone-11 and menaquinone-12. Physiological and biochemical characterization, phylogenetic analysis and DNA-DNA relatedness indicated that these two organisms are new species of the genus Microbacterium, for which the name Microbacterium kitamiense is proposed. The type strain of M. kitamiense is strain Kitami C2^T (= JCM 10270^T).

Key Words: Microbacterium kitamiense • polysaccharide • industrial wastewater

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences reported in this paper are AB013907 (Kitami C2^T) and AB013920 (Kitami A1).

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