| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB/ESIL Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France
2 Departamento de Biologia, Pontificia Universidad Javeriana, POB 56710, SantaFe de Bogota, Colombia
3 Département de Biologie, Université de la Mediterranée, 13288 Marseille Cedex 09, France
4 School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Nathan, Brisbane 4111, Australia
Author for correspondence: B. K. C. Patel. Tel: +61 417 726 671. Fax: +61 7 3875 7656. e-mail: bharat{at}genomes.sci.gu.edu.au
ABSTRACT
A novel, asaccharolytic, amino-acid-degrading bacterium, designated strain GLU-3T, was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. Strain GLU-3T stained Gram-negative and was an obligately anaerobic, non-spore-forming, slightly curved, rod-shaped bacterium (0·3 x 4·0–6·0 µm) which existed singly or in pairs. The DNA G+C content was 43 mol%. Optimum growth occurred at 35 °C and pH 7·5 on arginine with a generation time of 16 h. Good growth was obtained on arginine, histidine, threonine and glycine. Acetate was the end-product formed from all these substrates, but in addition, a trace of formate was detected from arginine and histidine, and ornithine was produced from arginine. Strain GLU-3T grew slowly on glutamate and produced acetate, carbon dioxide, formate, hydrogen and traces of propionate as the end-products. In syntrophic association with Methanobacterium formicicum, strain GLU-3T oxidized arginine, histidine and glutamate to give propionate as the major product; acetate, carbon dioxide and methane were also produced. Strain GLU-3T did not degrade alanine and the branched-chain amino acids valine, leucine and isoleucine either in pure culture or in association with M. formicicum. The nearest phylogenetic relative of strain GLU-3T was the thermophile Selenomonas acidaminovorans (similarity value of 89·5%). As strain GLU-3T is phylogenetically, physiologically and genotypically different from other amino-acid-degrading genera, it is proposed that it should be designated a new species of a new genus Aminomonas paucivorans gen. nov., sp. nov. (DSM 12260T).
Key Words: Aminomonas paucivorans • anaerobic bacterium • amino acid degradation • mixed culture • Methanobacterium formicicum
The GenBank accession numbers for the rRNA gene sequences of Aminomonas paucivorans strain GLU-3T, ‘Selenomonas acidaminovorans’ and ‘Selenomonas acidaminophila’ are AF072581, AF071414 and AF071415, respectively.
This article has been cited by other articles:
|
|
 |
|
  J. Downes, S. R. Vartoukian, F. E. Dewhirst, J. Izard, T. Chen, W.-H. Yu, I. C. Sutcliffe, and W. G. Wade Pyramidobacter piscolens gen. nov., sp. nov., a member of the phylum 'Synergistetes' isolated from the human oral cavity Int J Syst Evol Microbiol, May 1, 2009; 59(5): 972 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Jumas-Bilak, L. Roudiere, and H. Marchandin Description of 'Synergistetes' phyl. nov. and emended description of the phylum 'Deferribacteres' and of the family Syntrophomonadaceae, phylum 'Firmicutes' Int J Syst Evol Microbiol, May 1, 2009; 59(5): 1028 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ganesan, S. Chaussonnerie, A. Tarrade, C. Dauga, T. Bouchez, E. Pelletier, D. Le Paslier, and A. Sghir Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum 'Synergistetes', isolated from an anaerobic sludge digester Int J Syst Evol Microbiol, September 1, 2008; 58(9): 2003 - 2012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Diaz, S. Baena, M.-L. Fardeau, and B. K. C. Patel Aminiphilus circumscriptus gen. nov., sp. nov., an anaerobic amino-acid-degrading bacterium from an upflow anaerobic sludge reactor Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1914 - 1918. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-P. Horz, D. M. Citron, Y. A. Warren, E. J. C. Goldstein, and G. Conrads Synergistes group organisms of human origin. J. Clin. Microbiol., August 1, 2006; 44(8): 2914 - 2920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. E. Diaz, A. J. M. Stams, R. Amils, and J. L. Sanz Phenotypic properties and microbial diversity of methanogenic granules from a full-scale upflow anaerobic sludge bed reactor treating brewery wastewater. Appl. Envir. Microbiol., July 1, 2006; 72(7): 4942 - 4949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Dahle and N.-K. Birkeland Thermovirga lienii gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, amino-acid-degrading bacterium isolated from a North Sea oil well. Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1539 - 1545. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
