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1 Laboratoire ORSTOM de Microbiologie des Anaérobies, Université de Provence, CESB/ESIL Case 925, 163 Avenue de Luminy, 13288 Marseille Cedex 09, France
2 Departamento de Biologia, Pontificia Universidad Javeriana, POB 56710, SantaFe de Bogota, Colombia
3 Département de Biologie, Université de la Mediterranée, 13288 Marseille Cedex 09, France
4 School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Nathan, Brisbane 4111, Australia
Author for correspondence: B. K. C. Patel. Tel: +61 417 726 671. Fax: +61 7 3875 7656. e-mail: bharat{at}genomes.sci.gu.edu.au
ABSTRACT
A novel, asaccharolytic, amino-acid-degrading bacterium, designated strain GLU-3T, was isolated from an anaerobic lagoon of a dairy wastewater treatment plant. Strain GLU-3T stained Gram-negative and was an obligately anaerobic, non-spore-forming, slightly curved, rod-shaped bacterium (0·3 x 4·0–6·0 µm) which existed singly or in pairs. The DNA G+C content was 43 mol%. Optimum growth occurred at 35 °C and pH 7·5 on arginine with a generation time of 16 h. Good growth was obtained on arginine, histidine, threonine and glycine. Acetate was the end-product formed from all these substrates, but in addition, a trace of formate was detected from arginine and histidine, and ornithine was produced from arginine. Strain GLU-3T grew slowly on glutamate and produced acetate, carbon dioxide, formate, hydrogen and traces of propionate as the end-products. In syntrophic association with Methanobacterium formicicum, strain GLU-3T oxidized arginine, histidine and glutamate to give propionate as the major product; acetate, carbon dioxide and methane were also produced. Strain GLU-3T did not degrade alanine and the branched-chain amino acids valine, leucine and isoleucine either in pure culture or in association with M. formicicum. The nearest phylogenetic relative of strain GLU-3T was the thermophile Selenomonas acidaminovorans (similarity value of 89·5%). As strain GLU-3T is phylogenetically, physiologically and genotypically different from other amino-acid-degrading genera, it is proposed that it should be designated a new species of a new genus Aminomonas paucivorans gen. nov., sp. nov. (DSM 12260T).
Key Words: Aminomonas paucivorans • anaerobic bacterium • amino acid degradation • mixed culture • Methanobacterium formicicum
The GenBank accession numbers for the rRNA gene sequences of Aminomonas paucivorans strain GLU-3T, ‘Selenomonas acidaminovorans’ and ‘Selenomonas acidaminophila’ are AF072581, AF071414 and AF071415, respectively.
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