HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Aranaz, A. Articles by Domínguez, L. Search for Related Content PubMed PubMed Citation Articles by Aranaz, A. Articles by Domínguez, L. Agricola Articles by Aranaz, A. Articles by Domínguez, L.

Mycobacterium tuberculosis subsp. caprae subsp. nov.: A taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain

¹ Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
² Servicio de Microbiología, Hospital Ramón y Cajal, 28034 Madrid, Spain
³ Australian Reference Laboratory for Bovine Tuberculosis, Agriculture Western Australia, South Perth 6151, Australia
⁴ Centro Nacional de Investigación Clínica y M. P., Hospital Carlos III, 28029 Madrid, Spain

Author for correspondence: Lucas Domínguez. Tel: +34 91 3943721. Fax: +34 91 3943908. e-mail: alaranaz{at}eucmax.sim.ucm.es

ABSTRACT

Isolates from the Mycobacterium tuberculosis complex cultured from caprine pathological tissue samples were biochemically and genetically characterized. The isolates were negative for nitrate reduction and niacin accumulation, they weakly hydrolysed Tween 80, were sensitive to pyrazinamide (50 µg ml^–1) and were resistant to 1 and 2 µg tiophene-2-carboxylic acid hydrazide ml^–1 but not to 5 or 10 µg tiophene-2-carboxylic acid hydrazide ml^–1. Sequencing of the pncA gene revealed a polymorphism characteristic of M. tuberculosis, whereas oxyR, katG and gyrA sequences were characteristic of Mycobacterium bovis. The fingerprinting patterns obtained with IS6110, direct repeats and polymorphic G+C-rich sequence-associated RFLP and direct variable repeat-spacer oligonucelotide typing (spoligotyping) segregated these isolates from the other members of the complex. The results of this testing, together with the repeated association of this micro-organism with goats, suggest that a new member of this taxonomic complex not matching any of the classical species had been identified. This unusual mycobacterium may play a role in the epidemiology of animal and human tuberculosis in Spain. The name Mycobacterium tuberculosis subsp. caprae subsp. nov. is proposed for these isolates. The type strain of Mycobacterium tuberculosis subsp. caprae subsp. nov. is gM-1^T (= CIP 105776^T).

Key Words: Mycobacterium tuberculosis subsp • caprae subsp. nov. • Mycobacterium tuberculosis complex • tuberculosis • goats

The EMBL accession number for the 16S rRNA gene sequence of strain gM-1^T is AJ131120.

This article has been cited by other articles:

				A. Somoskovi, J. Dormandy, L. M. Parsons, M. Kaswa, K. S. Goh, N. Rastogi, and M. Salfinger Sequencing of the pncA Gene in Members of the Mycobacterium tuberculosis Complex Has Important Diagnostic Applications: Identification of a Species-Specific pncA Mutation in "Mycobacterium canettii" and the Reliable and Rapid Predictor of Pyrazinamide Resistance J. Clin. Microbiol., February 1, 2007; 45(2): 595 - 599. [Abstract] [Full Text] [PDF]

				B. Mathema, N. E. Kurepina, P. J. Bifani, and B. N. Kreiswirth Molecular Epidemiology of Tuberculosis: Current Insights Clin. Microbiol. Rev., October 1, 2006; 19(4): 658 - 685. [Abstract] [Full Text] [PDF]

				F. Mathews, D. W Macdonald, G. M. Taylor, M. Gelling, R. A Norman, P. E Honess, R. Foster, C. M Gower, S. Varley, A. Harris, et al. Bovine tuberculosis (Mycobacterium bovis) in British farmland wildlife: the importance to agriculture Proc R Soc B, February 7, 2006; 273(1584): 357 - 365. [Abstract] [Full Text] [PDF]

				W. M. Prodinger, A. Brandstatter, L. Naumann, M. Pacciarini, T. Kubica, M. L. Boschiroli, A. Aranaz, G. Nagy, Z. Cvetnic, M. Ocepek, et al. Characterization of Mycobacterium caprae Isolates from Europe by Mycobacterial Interspersed Repetitive Unit Genotyping J. Clin. Microbiol., October 1, 2005; 43(10): 4984 - 4992. [Abstract] [Full Text] [PDF]

				M. Ocepek, M. Pate, M. Zolnir-Dovc, and M. Poljak Transmission of Mycobacterium tuberculosis from Human to Cattle J. Clin. Microbiol., July 1, 2005; 43(7): 3555 - 3557. [Abstract] [Full Text] [PDF]

				S. Mostowy, A. Onipede, S. Gagneux, S. Niemann, K. Kremer, E. P. Desmond, M. Kato-Maeda, and M. Behr Genomic Analysis Distinguishes Mycobacterium africanum J. Clin. Microbiol., August 1, 2004; 42(8): 3594 - 3599. [Abstract] [Full Text] [PDF]

				W. Erler, G. Martin, K. Sachse, L. Naumann, D. Kahlau, J. Beer, M. Bartos, G. Nagy, Z. Cvetnic, M. Zolnir-Dovc, et al. Molecular Fingerprinting of Mycobacterium bovis subsp. caprae Isolates from Central Europe J. Clin. Microbiol., May 1, 2004; 42(5): 2234 - 2238. [Abstract] [Full Text] [PDF]

				C. Viana-Niero, P. E. de Haas, D. van Soolingen, and S. C. Leao Analysis of genetic polymorphisms affecting the four phospholipase C (plc) genes in Mycobacterium tuberculosis complex clinical isolates Microbiology, April 1, 2004; 150(4): 967 - 978. [Abstract] [Full Text] [PDF]

				S. Mostowy, D. Cousins, and M. A. Behr Genomic Interrogation of the Dassie Bacillus Reveals It as a Unique RD1 Mutant within the Mycobacterium tuberculosis Complex J. Bacteriol., January 1, 2004; 186(1): 104 - 109. [Abstract] [Full Text] [PDF]

				A. Aranaz, D. Cousins, A. Mateos, and L. Dominguez Elevation of Mycobacterium tuberculosis subsp. caprae Aranaz et al. 1999 to species rank as Mycobacterium caprae comb. nov., sp. nov. Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1785 - 1789. [Abstract] [Full Text] [PDF]

				D. V. Cousins, R. Bastida, A. Cataldi, V. Quse, S. Redrobe, S. Dow, P. Duignan, A. Murray, C. Dupont, N. Ahmed, et al. Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov. Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1305 - 1314. [Abstract] [Full Text] [PDF]

				E. Tortoli Impact of Genotypic Studies on Mycobacterial Taxonomy: the New Mycobacteria of the 1990s Clin. Microbiol. Rev., April 1, 2003; 16(2): 319 - 354. [Abstract] [Full Text] [PDF]

				R. C. Huard, L. C. de Oliveira Lazzarini, W. R. Butler, D. van Soolingen, and J. L. Ho PCR-Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions J. Clin. Microbiol., April 1, 2003; 41(4): 1637 - 1650. [Abstract] [Full Text] [PDF]

				A. G. M. van der Zanden, K. Kremer, L. M. Schouls, K. Caimi, A. Cataldi, A. Hulleman, N. J. D. Nagelkerke, and D. van Soolingen Improvement of Differentiation and Interpretability of Spoligotyping for Mycobacterium tuberculosis Complex Isolates by Introduction of New Spacer Oligonucleotides J. Clin. Microbiol., December 1, 2002; 40(12): 4628 - 4639. [Abstract] [Full Text] [PDF]

				L. M. Parsons, R. Brosch, S. T. Cole, A. Somoskovi, A. Loder, G. Bretzel, D. van Soolingen, Y. M. Hale, and M. Salfinger Rapid and Simple Approach for Identification of Mycobacterium tuberculosis Complex Isolates by PCR-Based Genomic Deletion Analysis J. Clin. Microbiol., July 1, 2002; 40(7): 2339 - 2345. [Abstract] [Full Text] [PDF]

				W. M. Prodinger, A. Eigentler, F. Allerberger, M. Schonbauer, and W. Glawischnig Infection of Red Deer, Cattle, and Humans with Mycobacterium bovis subsp. caprae in Western Austria J. Clin. Microbiol., June 1, 2002; 40(6): 2270 - 2272. [Abstract] [Full Text] [PDF]

				R. Brosch, S. V. Gordon, M. Marmiesse, P. Brodin, C. Buchrieser, K. Eiglmeier, T. Garnier, C. Gutierrez, G. Hewinson, K. Kremer, et al. A new evolutionary scenario for the Mycobacterium tuberculosis complex PNAS, March 19, 2002; 99(6): 3684 - 3689. [Abstract] [Full Text] [PDF]

				M. C. Menendez, E. Palenque, M. C. Navarro, M. C. Nunez, M. J. Rebollo, and M. J. Garcia Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques J. Clin. Microbiol., December 1, 2001; 39(12): 4241 - 4246. [Abstract] [Full Text] [PDF]

				M. P. U. Sales, G. M. Taylor, S. Hughes, M. Yates, G. Hewinson, D. B. Young, and R. J. Shaw Genetic Diversity among Mycobacterium bovis Isolates: a Preliminary Study of Strains from Animal and Human Sources J. Clin. Microbiol., December 1, 2001; 39(12): 4558 - 4562. [Abstract] [Full Text] [PDF]

				N. Haddad, A. Ostyn, C. Karoui, M. Masselot, M. F. Thorel, S. L. Hughes, J. Inwald, R. G. Hewinson, and B. Durand Spoligotype Diversity of Mycobacterium bovis Strains Isolated in France from 1979 to 2000 J. Clin. Microbiol., October 1, 2001; 39(10): 3623 - 3632. [Abstract] [Full Text] [PDF]