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Nocardia uniformis nom. rev.

¹ Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
² Department of Microbiology, College of Natural Sciences and Research Center for Molecular Biology, Seoul National University, Seoul 151–742, Republic of Korea

Author for correspondence: Michael Goodfellow. Tel: +44 191 222 7706. Fax: +44 191 222 5228. e-mail: m.goodfellow{at}ncl.ac.uk

ABSTRACT

A soil isolate representing the putatively novel species ‘Nocardia uniformis’ was found to have morphological, staining and chemotaxonomic properties consistent with its classification in the genus Nocardia. An almost complete sequence of the 16S rDNA of the strain was determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organism was consistently associated with the type strain of Nocardia otitidiscaviarum albeit with a relatively low bootstrap value recorded for neighbour-joining analysis. The strain was also readily separated from representatives of all validly described Nocardia species using a set of phenotypic properties. The genotypic and phenotypic data indicate that the strain should be assigned to the genus Nocardia as a new species. The name proposed for the new species is Nocardia uniformis. The type strain is JCM 3224^T.

Key Words: Nocardia uniformis nom. rev. • polyphasic taxonomy • 16S rDNA sequencing

The GenBank accession number for the 16S rDNA sequence of strain JCM 3224^T is Z46752.

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