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Pseudomonas libanensis sp. nov., a new specie isolated from Lebanese spring waters

¹ Laboratoire de Bactériologie-Hygiène, Faculté de Médecine Henri Warembourg, (Pôle Recherche), 1 Place de Verdun, 59045 Lille, France
² Faculté de Santé Publique, Section 3, Université Libanaise & CNRS Liban, France

Author for correspondence: H. Leclerc. Tel: +33 03 20 52 94 28. Fax: +33 03 20 52 93 61. e-mail: leclerc{at}univ-lille2.fr

ABSTRACT

The taxonomic position of eight fluorescent Pseudomonas isolates, from two Lebanese spring waters, which were previously recognized by numerical analysis as members of a new subcluster (subcluster Vb) was examined. Excep for one strain, the new subcluster exhibited internal DNA hybridization values of 76-100%, and 9–53% hybridization was measured with the type or reference strains of other Pseudomonas species. The highest DNA binding value was found with Pseudomonas marginalis strains (37–53%). The G+C content of the DNA of the type strain was 58 mol%. A comparison of 1322 nt of the 16S rRNA gene sequence of the strain representing subcluster Vb (CFML 96–1951^T) with the sequence of other strains of the genus Pseudomonas revealed that strain CFML 96–195^T was part of the ‘Pseudomonas fluorescens intrageneric cluster’. On the basis of the results of phenotypic, DNA-DNA and phylogenetic analyses, a new Pseudomonas species, Pseudomonas libanensis sp. nov., is proposed for the seven strains of subcluster Vb. The type strain is P. libanensis CFML 96–195^T and has been deposited in the Collection de I'lnstitut Pasteur (Paris, France) as CIP 105460^T. The P. libanensis strains are phenotypically and genotypically homogeneous and can be differentiated from most other fluorescent species by several phenotypic features. Differentiation of P. libanensis and Pseudomonas aeruginosa is based mainly on pyocyanin production; P. libanensis can be differentiated from P. fluorescens (all biovars) by -aminobutyrate assimilation. The clinical significance of P. libanensis is unknown.

Key Words: Pseudomonas libanensis sp. nov. • DNA-DNA hybridization • 16S rRNA

The EMBL accession number for the 16S rRNA gene sequence of strain CFML 96-195^T is AF057645.

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