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Molecular identification of Lactobacillus hilgardii and genetic relatedness with Lactobacillus brevis

Faculté d'ologie-Unité associée INRA-Université Victor Ségalen-Bordeaux II, 351 Cours de la Libération, 33405 Talence Cédex, France

Author for correspondence: Aline Lonvaud-Funel. Tel: +33 5 56 84 64 66. Fax: +33 5 56 84 64 68. e-mail: aline.lonvaud{at}oenologie.u-bordeaux2.fr

ABSTRACT

Conventional phenotypic methods lead to misidentification of the lactic acid bacteria Lactobacillus hilgardii and Lactobacillus brevis. Random amplified polymorphic DNA (RAPD) and repetitive element PCR (REP-PCR) techniques were developed for a molecular study of these two species. The taxonomic relationships were confirmed by analysis of the ribosomal operon. Amplified DNA fragments were chosen to isolate L. hilgardii-specific probes. In addition to rapid molecular methods for identification of L. hilgardii, these results convincingly proved that some strains first identified as L. brevis must be reclassified as L. hilgardii. The data clearly showed that these molecular methods are more efficient than phenotypic or biochemical studies for bacterial identification at the species level.

Key Words: Lactobacillus hilgardii • Lactobacillus brevis • RAPD • REP-PCR

The GenBank accession number for the 1. Rep probe sequence reported in this paper is AJ006299.

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