HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Padden, A. N. Articles by John, P. Search for Related Content PubMed PubMed Citation Articles by Padden, A. N. Articles by John, P. Agricola Articles by Padden, A. N. Articles by John, P.

An indigo-reducing moderate thermophile from a woad vat, Clostridium isatidis sp. nov.

A. Nikki Padden¹, Vivian M. Dillon¹, John Edmonds², M. David Collins^3,, Nerea Alvarez³ and Philip John¹

¹ Department of Agricultural Botany, University of Reading, Reading RG6 6AS, UK
² Chiltern Open Air Museum, Chalfont St Giles HP8 4AD, UK
³ Department of Microbiology, BBSRC Institute of Food Research, Reading Laboratory, Reading RG6 6AP, UK

Author for correspondence: A. Nikki Padden. Tel: +44 118 931 8098. Fax: +44 118 931 6577. e-mail: a.n.padden{at}reading.ac.uk

ABSTRACT

A Gram-positive, anaerobic, moderate thermophile, strain Wv6^T, capable of reducing indigo dye, was isolated from a fermenting woad vat prepared essentially as in medieval Europe. Strain Wv6^T formed rod-shaped cells, which occurred singly, in pairs or in chains and produced terminal oval endospores Strain Wv6^T was saccharolytic. Growth occurred at pH 5.9-9.9 (initial pH) with an optimum at 50 °C of pH 7.2±0.2 (constant pH). At pH 7.8, the temperature range for growth was 30-55 °C with the optimum at 49-52 °C. Comparative 16S rRNA gene sequence analysis demonstrated that the bacterium represents a hitherto unknown subline within rRNA cluster I Clostridium. Based on the results of the phylogenetic analysis and phenotypic criteria, it is proposed that the unknown moderate thermophile should be classified as Clostridium isatidi sp. nov., a new species of the genus Clostridium. The type strain of Clostridium isatidis is strain Wv6^T (= NCFB 3071^T).

Key Words: Clostridium isatidis sp. nov. • phylogeny • indigo reduction • moderate thermophile

The GenBank accession number for the 16S rRNA gene sequence of strain Wv6^T (=NCFB 3071^T isX98395.

Present address: Department of Food Science and Technology, University of Reading, Reading RG6 6AS, UK.

This article has been cited by other articles:

				I. Yumoto, K. Hirota, Y. Nodasaka, Y. Tokiwa, and K. Nakajima Alkalibacterium indicireducens sp. nov., an obligate alkaliphile that reduces indigo dye Int J Syst Evol Microbiol, April 1, 2008; 58(4): 901 - 905. [Abstract] [Full Text] [PDF]

				K. Nakajima, K. Hirota, Y. Nodasaka, and I. Yumoto Alkalibacterium iburiense sp. nov., an obligate alkaliphile that reduces an indigo dye Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1525 - 1530. [Abstract] [Full Text] [PDF]

				I. Yumoto, K. Hirota, Y. Nodasaka, Y. Yokota, T. Hoshino, and K. Nakajima Alkalibacterium psychrotolerans sp. nov., a psychrotolerant obligate alkaliphile that reduces an indigo dye Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2379 - 2383. [Abstract] [Full Text] [PDF]