| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Centers for Disease Control and Prevention, Bacterial & Mycotic Diseases, Mailstop G-05, 1600 Clifton Road, Atlanta, GA 30333, USA
2Viral & Rickettsial Diseases, Mailstop G-13, 1600 Clifton Road, Atlanta, GA 30333, USA
Author for correspondence: Eric L. Marston. Tel: +1 404 639 3929. Fax: +1 404 639 3970. e-mail: efm4{at}cdc.gov
ABSTRACT
A phylogenetic investigation was done on the members of the genus Bartonella, based on the DNA sequence analysis of the groEL gene, which encodes the 60 kDa heat-shock protein GroEL. Nucleotide sequence data were determined for a near full-length fragment (1368 bp) of the groEL gene of the established Bartonella species and used to infer intraspecies phylogenetic relationships. Phylogenetic trees were inferred from multiple sequence alignments by using both distance and parsimony methods, which demonstrated an architecture composed of six well-supported lineages. The results are consistent with relationships deduced from recent sequence analysis studies based upon citrate synthase (gltA) and previously observed genotypic and phenotypic characteristics; however, they showed greater statistical support at the intragenus level. This suggests that groEL may be a more robust tool for phylogenetic analysis of Bartonella lineages.
Key Words: Bartonella • groEL • 16S rRNA • maximum-likelihood • parsimony analysis
The GenBank accession numbers for the groEL sequences detemined in this paper are AF014830-AF014836.
This article has been cited by other articles:
|
|
 |
|
  H.-J. Chen, J.-C. Tsai, T.-C. Chang, W.-C. Hung, S.-P. Tseng, P.-R. Hsueh, and L.-J. Teng PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences J. Med. Microbiol., April 1, 2008; 57(4): 432 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Maggi and E. B. Breitschwerdt Potential Limitations of the 16S-23S rRNA Intergenic Region for Molecular Detection of Bartonella Species J. Clin. Microbiol., March 1, 2005; 43(3): 1171 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-C. Tsai, P.-R. Hsueh, H.-M. Lin, H.-J. Chang, S.-W. Ho, and L.-J. Teng Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region J. Clin. Microbiol., January 1, 2005; 43(1): 235 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. E. Hill, S. L. Penny, K. G. Crowell, S. H. Goh, and S. M. Hemmingsen cpnDB: A Chaperonin Sequence Database Genome Res., August 1, 2004; 14(8): 1669 - 1675. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. B. Chomel, A. C. Wey, and R. W. Kasten Isolation of Bartonella washoensis from a Dog with Mitral Valve Endocarditis J. Clin. Microbiol., November 1, 2003; 41(11): 5327 - 5332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hoffmann and A. Roggenkamp Population Genetics of the Nomenspecies Enterobacter cloacae Appl. Envir. Microbiol., September 1, 2003; 69(9): 5306 - 5318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Zhu, W. Li, and X. Dong Species identification of genus Bifidobacterium based on partial HSP60 gene sequences and proposal of Bifidobacterium thermacidophilum subsp. porcinum subsp. nov. Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1619 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Lee, H.-S. Park, W.-J. Jang, S.-E. Koh, J.-M. Kim, S.-K. Shim, M.-Y. Park, Y.-W. Kim, B.-J. Kim, Y.-H. Kook, et al. Differentiation of Rickettsiae by groEL Gene Analysis J. Clin. Microbiol., July 1, 2003; 41(7): 2952 - 2960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Johnson, M. Ayers, S. C. C. McClure, S. E. Richardson, and R. Tellier Detection and Identification of Bartonella Species Pathogenic for Humans by PCR Amplification Targeting the Riboflavin Synthase Gene (ribC) J. Clin. Microbiol., March 1, 2003; 41(3): 1069 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kosoy, M. Murray, R. D. Gilmore Jr., Y. Bai, and K. L. Gage Bartonella Strains from Ground Squirrels Are Identical to Bartonella washoensis Isolated from a Human Patient J. Clin. Microbiol., February 1, 2003; 41(2): 645 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Dillon, J. Valenzuela, R. Don, D. Blanckenberg, D. I. Wigney, R. Malik, A. J. Morris, J. M. Robson, and J. Iredell Limited Diversity among Human Isolates of Bartonella henselae J. Clin. Microbiol., December 1, 2002; 40(12): 4691 - 4699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zeaiter, Z. Liang, and D. Raoult Genetic Classification and Differentiation of Bartonella Species Based on Comparison of Partial ftsZ Gene Sequences J. Clin. Microbiol., October 1, 2002; 40(10): 3641 - 3647. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Teng, P.-R. Hsueh, J.-C. Tsai, P.-W. Chen, J.-C. Hsu, H.-C. Lai, C.-N. Lee, and S.-W. Ho groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group Streptococci J. Clin. Microbiol., September 1, 2002; 40(9): 3172 - 3178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Zeaiter, P.-E. Fournier, and D. Raoult Genomic Variation of Bartonella henselae Strains Detected in Lymph Nodes of Patients with Cat Scratch Disease J. Clin. Microbiol., March 1, 2002; 40(3): 1023 - 1030. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Jacomo, P. J. Kelly, and D. Raoult Natural History of Bartonella Infections (an Exception to Koch's Postulate) Clin. Vaccine Immunol., January 1, 2002; 9(1): 8 - 18. [Full Text] [PDF]
|
|
|
|
|
|
L.-J. Teng, P.-R. Hsueh, Y.-H. Wang, H.-M. Lin, K.-T. Luh, and S.-W. Ho Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification J. Clin. Microbiol., September 1, 2001; 39(9): 3326 - 3331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Houpikian and D. Raoult 16S/23S rRNA Intergenic Spacer Regions for Phylogenetic Analysis, Identification, and Subtyping of Bartonella Species J. Clin. Microbiol., August 1, 2001; 39(8): 2768 - 2778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Drancourt, A. Carlioz, and D. Raoult rpoB Sequence Analysis of Cultured Tropheryma whippelii J. Clin. Microbiol., July 1, 2001; 39(7): 2425 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sander, A. Zagrosek, W. Bredt, E. Schiltz, Y. Piémont, C. Lanz, and C. Dehio Characterization of Bartonella clarridgeiae Flagellin (FlaA) and Detection of Antiflagellin Antibodies in Patients with Lymphadenopathy J. Clin. Microbiol., August 1, 2000; 38(8): 2943 - 2948. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Handley and R. L. Regnery Differentiation of Pathogenic Bartonella Species by Infrequent Restriction Site PCR J. Clin. Microbiol., August 1, 2000; 38(8): 3010 - 3015. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
