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1Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
2Department of Microbiology, College of Natural Sciences and Research Center for Molecular Biology, Seoul National University, Seoul 151-742, Republic of Korea
Author for correspondence: Michael Goodfellow. Tel: +44 191 222 7706. Fax: +44 191 222 5228. e-mail: m.goodfellow{at}ncl.ac.uk
ABSTRACT
An almost complete gene sequence of 16S rDNA of Nocardia salmonicida strain JCM 4826Twas determined following cloning and sequencing of the amplified gene. The sequence was aligned with those available for nocardiae and phylogenetic trees inferred using four tree-making algorithms. The organism and the type strain of Nocardia asteroides consistently formed a monophyletic clade with a distant sequence similarity of 97%. However, previous DNA relatedness experiments showed that strain JCM 4826Tand Nocardia asteroides ATCC 19247Tbelong to different genomic species. The organism was also distinguished from representatives of all validly described species of Nocardia using a combination of phenotypic features. The polyphasic evidence showed that the strain merits recognition as a new species of the genus Nocardia. The name proposed for the new species is Nocardia salmonicida nom. rev.
Key Words: Nocardia salmonicida nom. rev. polyphasic taxonomy 16S rDNA sequencing
The GenBank accession number for the 16S rDNA sequence of strain JCM 4826T is Z46750.
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