| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Virology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel
2Avian and Swine Respiratory Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, US Department of Agriculture, Ames, IA 50010, USA
Author for correspondence: Maureen G. Friedman. Tel: +972 7 640 0867. Fax: +972 7 627 6215. e-mail: maureen{at}bgumail.bgu.ac.il
ABSTRACT
Simkania negevensis is the type species of Simkaniaceae, a recently proposed family in the order Chlamydiales. In the current study, growth, antigenic and genomic characteristics of this intracellular bacterium were investigated and compared to those of members of the family Chlamydiaceae. Growth of the organism, as assessed by infectivity assays, reached a plateau in 2--3 d although by light microscopy the cytopathic effect on the host cells increased for 12 or more days after infection. S. negevensis growth was unaffected by sulfadiazine. Cells infected by S. negevensis strain ZTwere not recognized by either of two monoclonal antibodies specific for Chlamydiaceae LPS and several specific Chlamydiaceae ompA primers were unable to PCR amplify a S. negevensis gene. The S. negevensis genome contained one copy of the ribosomal operon. The genome size of S. negevensis strain ZTwas determined by PFGE to be 1·7 Mbp, and the G+C content was 42·5 mol%. These data, taken together with other published data, are consistent with the proposal that S. negevensis belongs to a distinct family in the order Chlamydiales.
Key Words: Chlamydiales • Simkaniaceae • rDNA copy number • genome size • Chlamydia
The GenBank accession number for the 23S rRNA sequence of Simkania negevensis strain ZTis U68460
Present address: Department of Medical Microbiology and Parasitology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602--7371, USA.
This article has been cited by other articles:
|
|
 |
|
  N. Casson, J. M. Entenza, and G. Greub Serological Cross-Reactivity between Different Chlamydia-Like Organisms J. Clin. Microbiol., January 1, 2007; 45(1): 234 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M G Friedman, S Kahane, B Dvoskin, and J W Hartley Detection of Simkania negevensis by culture, PCR, and serology in respiratory tract infection in Cornwall, UK. J. Clin. Pathol., March 1, 2006; 59(3): 331 - 333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Griffiths, A. K. Petrich, and R. S. Gupta Conserved indels in essential proteins that are distinctive characteristics of Chlamydiales and provide novel means for their identification Microbiology, August 1, 2005; 151(8): 2647 - 2657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Draghi II, V. L. Popov, M. M. Kahl, J. B. Stanton, C. C. Brown, G. J. Tsongalis, A. B. West, and S. Frasca Jr. Characterization of "Candidatus Piscichlamydia salmonis" (Order Chlamydiales), a Chlamydia-Like Bacterium Associated With Epitheliocystis in Farmed Atlantic Salmon (Salmo salar) J. Clin. Microbiol., November 1, 2004; 42(11): 5286 - 5297. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Soldati, Z. H. Lu, L. Vaughan, A. Polkinghorne, D. R. Zimmermann, J. B. Huder, and A. Pospischil Detection of Mycobacteria and Chlamydiae in Granulomatous Inflammation of Reptiles: A Retrospective Study Vet. Pathol., July 1, 2004; 41(4): 388 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kahane, N. Platzner, B. Dvoskin, A. Itzhaki, and M. G. Friedman Evidence for the Presence of Simkania negevensis in Drinking Water and in Reclaimed Wastewater in Israel Appl. Envir. Microbiol., June 1, 2004; 70(6): 3346 - 3351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Poppert, A. Essig, R. Marre, M. Wagner, and M. Horn Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization Appl. Envir. Microbiol., August 1, 2002; 68(8): 4081 - 4089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Griffiths and R. S. Gupta Protein signatures distinctive of chlamydial species: horizontal transfers of cell wall biosynthesis genes glmU from archaea to chlamydiae and murA between chlamydiae and Streptomyces Microbiology, August 1, 2002; 148(8): 2541 - 2549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahane, N. Kimmel, and M. G. Friedman The growth cycle of Simkania negevensis Microbiology, March 1, 2002; 148(3): 735 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kahane, B. Dvoskin, M. Mathias, and M. G. Friedman Infection of Acanthamoeba polyphaga with Simkania negevensis and S. negevensis Survival within Amoebal Cysts Appl. Envir. Microbiol., October 1, 2001; 67(10): 4789 - 4795. [Abstract] [Full Text]
|
|
|
|
|
|
M. Horn, M. Wagner, K.-D. Müller, E. N. Schmid, T. R. Fritsche, K.-H. Schleifer, and R. Michel Neochlamydia hartmannellae gen. nov., sp. nov. (Parachlamydiaceae), an endoparasite of the amoeba Hartmannella vermiformis Microbiology, May 1, 2000; 146(5): 1231 - 1239. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. D. E. Everett, S. Kahane, R. M. Bush, and M. G. Friedman An Unspliced Group I Intron in 23S rRNA Links Chlamydiales, Chloroplasts, and Mitochondria J. Bacteriol., August 15, 1999; 181(16): 4734 - 4740. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
