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Streptococcus waius sp. nov., a thermophilic streptococcus from a biofilm

¹Institute of Food Nutrition and Human Health, Massey University, Private Bag 11222, Palmerston North, New Zealand
²New Zealand Dairy Research Institute, Private Bag 11029, Palmerston North, New Zealand

Author for correspondence: Steve H. Flint. Tel: +64 6 350 4649. Fax: +64 6 356 1476. e-mail: steve.flint@nzdri.org.nz

ABSTRACT

Thermophilic streptococci were isolated from biofilms on stainless steel samples exposed to pasteurized skimmed milk and from dairy products from a dairy manufacturing plant. The phenotypic characters of these isolates were distinct from those of other thermophilic streptococci of dairy origin (Streptococcus thermophilus and Streptococcus bovis). Genotypic data [restriction endonuclease analysis, ribotyping, random amplified polymorphic DNA (RAPD) profiles, DNA-DNA hybridization and G+C contents] support the classification of these isolates as a new species. The sequence of the 16S rRNA was compared with that of 29 species of streptococci and shown to be significantly different. The sequence of the 16S-23S rRNA intergenic spacer region also differed from published sequences of closely related species. A fluorescent in situ hybridization probe prepared to a specific part of the 16S rRNA gene sequence was able to distinguish the unknown isolates from reference isolates of S. thermophilus and S. bovis. It is proposed that these thermophilic streptococcal isolates from a dairy environment be classified in the genus Streptococcus as a new species, Streptococcus waius (from waiu, the New Zealand Maori word for milk). The type strain is 3/1^T(= NZRCC 20100^T).

Key Words: Streptococcus thermophilus • Streptococcus waius • biofilms • milk

The GenBank accession numbers for the partial 16S rRNA and complete 16S rRNA sequences and the 16S-23S intergenic spacer region sequence determined in this work are U96621, AF088900 and AF088899, respectively.

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