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Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children's day care centre

¹Institut für Angewandte Mikrobiologie, Justus-Liebig Universität, Senckenbergstr. 3, D-35390 Giessen, Germany
²Department of Applied Chemistry and Microbiology, PO Box 56 (Biocentre), 00014 University of Helsinki, Finland
³Department of Biological Sciences, 508 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA
⁴DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, D-38124 Braunschweig, Germany

Author for correspondence: Peter Kämpfer. Tel: +49 641 99 37352. Fax: +49 641 99 37359. e-mail: peter.kaempfer{at}agrar.uni-giessen.de

ABSTRACT

The taxonomic status of an actinomycete (MA140/96^T) isolated from indoor building materials of a children's day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positi bacteria. The organisms grew at 10--37°C no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid meso-diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK9(H²). The fatty acid pattern was composed of palmitic acid (23.6%) palmitole acid (16.5%) and another hexadecenoic acid 16:1cis11 (1.4%), oleic acid (29.9%), stearic acid (2.9%) and the 10-methyl-branched tuberculostearic acid (23.2%). A gas-chromatographic analysis of the mycolic acid revealed a carbo chain length of C⁵⁰-C⁵⁶The G+C was 64.8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96^Trepresents a new lineage in the suborder Corynebacterineae of the order Actinomycetales. Therefore, it was concluded that strain MA140/96^Tshould be assigned to a new genus and species, for which the name Williamsia muralis gen. nov., sp. nov. proposed. The type strain of the species is MA140/96^T(= DSM 44343^T).

Key Words: Williamsia muralis gen. nov. • sp. nov. • actinomycete • indoor contaminant

The EMBL accession number for the 16S rDNA sequence of strain MA140/96^Tis Y17384.

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