| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Gent, University of Ghent, Belgium
2BCCM/LMG Culture Collection, University of Ghent, Belgium
3Department of Clinical Bacteriology, University of Göteborg, S-413 46 Göteborg, Sweden
Author for correspondence: P. Vandamme. Tel: +32 9 264 51 13. Fax: +32 9 264 50 92. e-mail: peter.vandamme{at}rug.ac.be
ABSTRACT
An integrated genotypic and phenotypic analysis of 12 Centers for Disease Control (CDC) group IVc-2 strains revealed that this taxon represents a novel species belonging to the genus Ralstonia. Comparative 16S rDNA sequence analysis allocated a representative CDC group IVc-2 strain to the Ralstonia branch of the β subclass of the Proteobacteria. DNA-DNA hybridizations did not detect significant binding levels towards any presently known Ralstonia species, including Ralstonia pickettii. Its DNA base ratio is between 65 and 67 mol%. The name Ralstonia paucula sp. nov. is proposed, with strain LMG 3244 (= CDC E6793), isolated from a human respiratory tract, as the type strain. R. paucula can be differentiated from other Ralstonia species by whole-cell protein analysis, amplified rDNA restriction analysis and a variety of classical biochemical tests. Strains have been isolated from various human clinical and environmental sources.
Key Words: CDC group IVc-2 • Ralstonia • β-Proteobacteria
The GenBank/EMBL/DDBJ accession number for the 16S rDNA sequence of strain LMG 3413Tis AF085226.
This article has been cited by other articles:
|
|
 |
|
  Y. Sato, H. Nishihara, M. Yoshida, M. Watanabe, J. D. Rondal, R. N. Concepcion, and H. Ohta Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines. Int J Syst Evol Microbiol, May 1, 2006; 56(Pt 5): 973 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Daxboeck, M. Stadler, O. Assadian, E. Marko, A. M Hirschl, and W. Koller Characterization of clinically isolated Ralstonia mannitolilytica strains using random amplification of polymorphic DNA (RAPD) typing and antimicrobial sensitivity, and comparison of the classification efficacy of phenotypic and genotypic assays J. Med. Microbiol., January 1, 2005; 54(1): 55 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Vandamme and T. Coenye Taxonomy of the genus Cupriavidus: a tale of lost and found Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2285 - 2289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Vaneechoutte, P. Kampfer, T. De Baere, E. Falsen, and G. Verschraegen Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol, March 1, 2004; 54(2): 317 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Moissenet, H. Vu-Thien, Y. Benzerara, and G. Arlet DNA Fingerprinting of Ralstonia paucula by Infrequent-Restriction-Site PCR and Randomly Amplified Polymorphic DNA Analysis J. Clin. Microbiol., December 1, 2003; 41(12): 5747 - 5749. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Coenye, P. Vandamme, and J. J. LiPuma Ralstonia respiraculi sp. nov., isolated from the respiratory tract of cystic fibrosis patients Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1339 - 1342. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Coenye, J. Goris, P. De Vos, P. Vandamme, and J. J. LiPuma Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1075 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Brisse, S. Stefani, J. Verhoef, A. Van Belkum, P. Vandamme, and W. Goessens Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex J. Clin. Microbiol., May 1, 2002; 40(5): 1743 - 1748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Laffineur, M. Janssens, J. Charlier, V. Avesani, G. Wauters, and M. Delmee Biochemical and Susceptibility Tests Useful for Identification of Nonfermenting Gram-Negative Rods J. Clin. Microbiol., March 1, 2002; 40(3): 1085 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Clermont, C. Harmant, and C. Bizet Identification of Strains of Alcaligenes and Agrobacterium by a Polyphasic Approach J. Clin. Microbiol., September 1, 2001; 39(9): 3104 - 3109. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Henry, E. Mahenthiralingam, P. Vandamme, T. Coenye, and D. P. Speert Phenotypic Methods for Determining Genomovar Status of the Burkholderia cepacia Complex J. Clin. Microbiol., March 1, 2001; 39(3): 1073 - 1078. [Abstract] [Full Text]
|
|
|
|
 |
|
 N.-Y. Zhou, S. L. Fuenmayor, and P. A. Williams nag Genes of Ralstonia (Formerly Pseudomonas) sp. Strain U2 Encoding Enzymes for Gentisate Catabolism J. Bacteriol., January 15, 2001; 183(2): 700 - 708. [Abstract] [Full Text]
|
|
|
|
|
|
D. Moissenet, P. Bidet, A. Garbarg-Chenon, G. Arlet, and H. Vu-Thien Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum) J. Clin. Microbiol., January 1, 2001; 39(1): 381 - 384. [Abstract] [Full Text]
|
|
|
|
|
|
P. Vandamme, E. Mahenthiralingam, B. Holmes, T. Coenye, B. Hoste, P. De Vos, D. Henry, and D. P. Speert Identification and Population Structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia Genomovar IV) J. Clin. Microbiol., March 1, 2000; 38(3): 1042 - 1047. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
