| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Station Biologique, UPR9042, Centre National de la Recherche Scientifique and Université Pierre et Marie Curie, Place Georges-Teissier, 29680 Roscoff, France
2Université de Bretagne Occidentale, UFR des Sciences et Techniques, 6 avenue Victor Le Gorgeu, 29285 Brest cedex, France
Author for correspondence: Christian Jeanthon. Tel: +33 298292309. Fax: +33 298292324. e-mail: jeanthon{at}sb-roscoff.fr
ABSTRACT
16S rDNAs amplified by PCR from 22 hyperthermophilic methanococci isolated from deep-sea hydrothermal vents were compared with those of the six type strains of the genus Methanococcus by RFLP analysis. Restriction fragments obtained with Haelll enabled four of the type species to be distinguished. Restrictions with Hhal, Bstul and Mspl were necessary to differentiate Methanococcus jannaschii and Methanococcus fervens. The results indicate that the 16S rDNA PCR-RFLP method provides a rapid and reliable tool for the identification of newly isolated hyperthermophilic Methanococcus spp.
Key Words: Methanococcus • RFLP • 16S rDNA • Archaea • hydrothermal vents
This article has been cited by other articles:
|
|
 |
|
  K. G. Lloyd, V. P. Edgcomb, S. J. Molyneaux, S. Boer, C. O. Wirsen, M. S. Atkins, and A. Teske Effects of Dissolved Sulfide, pH, and Temperature on Growth and Survival of Marine Hyperthermophilic Archaea Appl. Envir. Microbiol., October 1, 2005; 71(10): 6383 - 6387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dhillon, M. Lever, K. G. Lloyd, D. B. Albert, M. L. Sogin, and A. Teske Methanogen Diversity Evidenced by Molecular Characterization of Methyl Coenzyme M Reductase A (mcrA) Genes in Hydrothermal Sediments of the Guaymas Basin Appl. Envir. Microbiol., August 1, 2005; 71(8): 4592 - 4601. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Grabowski, B. J. Tindall, V. Bardin, D. Blanchet, and C. Jeanthon Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1113 - 1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takai, K. H. Nealson, and K. Horikoshi Methanotorris formicicus sp. nov., a novel extremely thermophilic, methane-producing archaeon isolated from a black smoker chimney in the Central Indian Ridge Int J Syst Evol Microbiol, July 1, 2004; 54(4): 1095 - 1100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L'Haridon, A.-L. Reysenbach, A. Banta, P. Messner, P. Schumann, E. Stackebrandt, and C. Jeanthon Methanocaldococcus indicus sp. nov., a novel hyperthermophilic methanogen isolated from the Central Indian Ridge Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1931 - 1935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takai, S. Nakagawa, Y. Sako, and K. Horikoshi Balnearium lithotrophicum gen. nov., sp. nov., a novel thermophilic, strictly anaerobic, hydrogen-oxidizing chemolithoautotroph isolated from a black smoker chimney in the Suiyo Seamount hydrothermal system Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1947 - 1954. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
