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1Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
2Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Göteborg, Sweden
3BCCM/LMG Culture Collection, Laboratorium voor Microbiologie Gent, Universiteit Gent, Belgium
4Department of Medical Microbiology, University of Edinburgh, Edinburgh, UK
5Department of Medical Microbiology, University Hospital UIA, Antwerp, Belgium
Author for correspondence: T. Coenye. Tel: +32 9 264 51 14. Fax: +32 9 264 50 92. e-mail: Tom.Coenye{at}rug.ac.be
ABSTRACT
A polyphasic taxonomic study that included DNA-DNA hybridizations, DNA base ratio determinations, 16S rDNA sequence analysis, whole-cell protein and fatty acid analyses, AFLP (amplified fragment length polymorphism) fingerprinting and an extensive biochemical characterization was performed on 10 strains provisionally identified as Alcaligenes faecalis-like bacteria. The six environmental and four human isolates belonged to the genus Ralstonia and were assigned to a new species for which the name Ralstonia gilardii sp. nov. is proposed. The type strain is LMG 5886T.
Key Words: Ralstonia gilardii sp. nov. • Ralstonia eutropha • taxonomy
The GenBank accession number for the fragment of the 16S rRNA gene of Rastonia gilardii sp. nov. is AF076645.
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