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1Molecular Ecology Group, Max-Planck-Institute for Marine Microbiology, Celsiusstraβe 1, D-28359 Bremen, Germany
2Department of Microbiology, Max-Planck-Institute for Marine Microbiology, Celsiusstraβe 1, D-28359 Bremen, Germany
3Netherlands Institute for Sea Research (NIOZ), PO Box 59, NL-1790 AB Den Burg (Texel), The Netherlands
4Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
Author for correspondence: Jan Kuever. Tel: +49 421 2028 734. Fax: +49 421 2028 580. e-mail: jkuever{at}mpi-bremen.de
ABSTRACT
Two new members of the genus Thiomicrospira were isolated from an intertidal mud flat sample with thiosulfate as the electron donor and CO2 as carbon source. On the basis of differences in genotypic and phenotypic characteristics, it is proposed that strain JB-A1T (= DSM 12350T) and strain JB-A2T (= DSM 12351T) are members of two new species, Thiomicrospira kuenenii and Thiomicrospira frisia, respectively. The cells were Gram-negative vibrios or slightly bent rods. Strain JB-A1T was highly motile, whereas strain JB-A2T showed a much lower degree of motility combined with a strong tendency to form aggregates. Both organisms were obligately autotrophic and strictly aerobic. Nitrate was not used as electron acceptor. Chemolithoautotrophic growth was observed with thiosulfate, tetrathionate, sulfur and sulfide. Neither isolate was able to grow heterotrophically. For strain JB-A1T, growth was observed between pH values of 4·0 and 7·5 with an optimum at pH 6·0, whereas for strain JB-A2T, growth was observed between pH 4·2 and 8·5 with an optimum at pH 6·5. The temperature limits for growth were between 3·5 and 42°C and 3·5 and 39 °C, respectively. The optimum growth temperature for strain JB-A1T was between 29 and 33·5 °C, whereas strain JB-A2T showed optimal growth between 32 and 35 °C. The mean maximum growth rate on thiosulfate was 0·35 h-1for strain JB-A1T and 0·45 h-1for strain JB-A2T.
Key Words: Thiomicrospira • sulfur-oxidizing bacteria • Wadden Sea
The GenBank accession numbers for the 16S rRNA sequences of strains JB-A1T and JB-A2T are AF013978 and AF013974, respectively.
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