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1Institute of Biotechnology, National Center for Natural Science and Technology, Nghia do, Tu liem, Hanoi, Vietnam
2Institut für Mikrobiologie und Genetik, Universität Wien, Dr Bohrgasse 9, A-1030 Wien, Austria
3Botanisches Institut der Universität München, Menzinger Str. 67, D-80638 München, Germany
4Institut für Genetik und Allgemeine Biologie, Hellbrunnerstr. 34, A-5020 Salzburg, Austria
Author for correspondence: Helga Stan-Lotter. Tel: +43 662 8044 5756. Fax: +43 662 8044 144. e-mail: helga.stan-lotter{at}sbg.ac.at
ABSTRACT
Several strains of moderately halophilic and mesophilic bacteria were isolated at the head of an oil-producing well on an offshore platform in southern Vietnam. Cells were Gram-negative, non-spore-forming, rod-shaped and motile by means of a polar flagellum. Growth occurred at NaCI concentrations between 0 and 20%; the optimum was 5% NaCI. One strain, which was designated VT8T, could degrade n-hexadecane, pristane and some crude oil components. It grew anaerobically in the presence of nitrate on succinate, citrate or acetate, but not on glucose. Several organic acids and amino acids were utilized as sole carbon and energy sources. The major components of its cellular fatty acids were C12:0 3-OH, C16:1 
9c, C16:0 and C18:1 9c. The DNA G+C content was 55·7 mol%. 16S rDNA sequence analysis indicated that strain VT8Twas closely related to Marinobacter sp. strain CAB (99·8% similarity) and Marinobacter hydrocarbonoclasticus (99·4% similarity). Its antibiotic resistance, isoprenoid quinones and fatty acids were similar to those of Marinobacter hydrocarbonoclasticus and Pseudomonas nautica. However, the whole-cell protein pattern of VT8Tdiffered from that of other halophilic marine isolates, including P. nautica. DNA-DNA hybridization indicated that the level of relatedness to Marinobacter hydrocarbonoclasticus was 65% and that to P. nautica was 75%. Further differences were apparent in Fourier-transformed IR spectra of cells and lipopolysaccharide composition. It is proposed that VT8Tshould be the type strain of a new species and should be named Marinobacter aquaeolei. P. nautica may have been misclassified, as suggested previously, and may also belong to the genus Marinobacter.
Key Words: Marinobacter aquaeolei • 16S rRNA gene analysis • Fourier-transformed IR spectroscopy • lipopolysaccharide • Pseudomonas nautica
The EMBL accession number for the sequence reported in this paper is J000726.
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