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Desulfobacca acetoxidans gen. nov., sp. nov., a novel acetate-degrading sulfate reducer isolated from sulfidogenic granular sludge

Stefanie J. W. H. Oude Elferink, W. M. Akkermans-van Vliet, Jaap J. Bogte and Alfons J. M. Stams

Department of Microbiology, Wageningen Agricultural University, H.v. Suchtelenweg 4, NL-6703 CT Wageningen, The Netherlands

Author for correspondence: Stefanie J. W. H. Oude Elferink. Tel: +31 320 237 359. Fax: +31 320 237 320. e-mail: s.j.w.h.oudeelferink{at}id.dlo.nl

ABSTRACT

A mesophilic sulfate reducer, strain ASRB2^T, was isolated with acetate as sole carbon and energy source from granular sludge of a laboratory-scale upflow anaerobic sludge bed reactor fed with acetate and sulfate. The bacterium was oval-shaped, 1·3 x 1·9-2·2 µm, non-motile and Gram-negative. Optimum growth with acetate occurred around 37°C in freshwater medium (doubling time: 1·7-2·2 d). Enzyme studies indicated that acetate was oxidized via the carbon monoxide dehydrogenase pathway. Growth was not supported by other organic acids, such as propionate, butyrate or lactate, alcohols such as ethanol or propanol, and hydrogen or formate. Sulfite and thiosulfate were also used as electron acceptors, but sulfur and nitrate were not reduced. Phylogenetically, strain ASRB2^Tclustered with the delta subclass of the Proteobacteria. Its closest relatives were Desulfosarcina variabilis, Desulfacinum infernum and Syntrophus buswellii. Strain ASRB2^Tis described as the type strain of Desulfobacca acetoxidans gen. nov., sp. nov.

Key Words: Desulfobacca acetoxidans gen. nov. • sp. nov. • acetate • sulfate-reducing bacteria

The GenBank accession number for the 16S rRNA gene sequence of strain ASRB2^Tis AF002671.

Present address: DLO-Institute for Animal Science and Health (ID-DLO), Department of Ruminant Nutrition, PO Box 65, 8200 AB Lelystad, The Netherlands.

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