| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark
2Institut für Medizinische Mikrobiologie, Klinikum der Philipps-Universtät, Pilgrimstein 2, D-35037 Marburg, Germany
3National Institute of Public Health, Department of Bacteriology, PO Box 4404 Torshov, 0403 Oslo, Norway
Author for correspondence: Øystein Angen. Fax: +45 35300120. e-mail: ang{at}svs.dk
ABSTRACT
The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] Haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as ‘biotype A’ of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov.
Key Words: Pasteurella haemolytica • Mannheimia gen. nov. • DNA-DNA hybridization • 16s rRNA sequencing • polyphasic taxonomy
The GenBank accession numbers for the sequences reported in this paper are listed in Table 1.
Present address: Danish Veterinary Laboratory, Department of Microbiology, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
This article has been cited by other articles:
|
|
 |
|
  J. L. George, D. J. Martin, P. M. Lukacs, and M. W. Miller EPIDEMIC PASTEURELLOSIS IN A BIGHORN SHEEP POPULATION COINCIDING WITH THE APPEARANCE OF A DOMESTIC SHEEP J. Wildl. Dis., April 1, 2008; 44(2): 388 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Bojesen, J. Larsen, A. G. Pedersen, T. Morner, R. Mattson, and M. Bisgaard IDENTIFICATION OF A NOVEL MANNHEIMIA GRANULOMATIS LINEAGE FROM LESIONS IN ROE DEER (CAPREOLUS CAPREOLUS) J. Wildl. Dis., July 1, 2007; 43(3): 345 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Blackall, A. M. Bojesen, H. Christensen, and M. Bisgaard Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi gen. nov., comb. nov. Int J Syst Evol Microbiol, April 1, 2007; 57(4): 666 - 674. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, P. Kuhnert, H.-J. Busse, W. C. Frederiksen, and M. Bisgaard Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae Int J Syst Evol Microbiol, January 1, 2007; 57(1): 166 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gioia, X. Qin, H. Jiang, K. Clinkenbeard, R. Lo, Y. Liu, G. E. Fox, S. Yerrapragada, M. P. McLeod, T. Z. McNeill, et al. The Genome Sequence of Mannheimia haemolytica A1: Insights into Virulence, Natural Competence, and Pasteurellaceae Phylogeny. J. Bacteriol., October 1, 2006; 188(20): 7257 - 7266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Norskov-Lauritsen and M. Kilian Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2135 - 2146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Kuhnert and B. M. Korczak Prediction of whole-genome DNA-DNA similarity, determination of G+C content and phylogenetic analysis within the family Pasteurellaceae by multilocus sequence analysis (MLSA). Microbiology, September 1, 2006; 152(Pt 9): 2537 - 2548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Safaee, G. C. Weiser, E. F. Cassirer, R. R. Ramey, and S. T. Kelley Microbial diversity in bighorn sheep revealed by culture-independent methods. J. Wildl. Dis., July 1, 2006; 42(3): 545 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-L. Gautier, D. Dubois, F. Escande, J.-L. Avril, P. Trieu-Cuot, and O. Gaillot Rapid and Accurate Identification of Human Isolates of Pasteurella and Related Species by Sequencing the sodA Gene J. Clin. Microbiol., May 1, 2005; 43(5): 2307 - 2314. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Blackall, H. Christensen, T. Beckenham, L. L. Blackall, and M. Bisgaard Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov. Int J Syst Evol Microbiol, January 1, 2005; 55(1): 353 - 362. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Norskov-Lauritsen, B. Bruun, and M. Kilian Multilocus sequence phylogenetic study of the genus Haemophilus with description of Haemophilus pittmaniae sp. nov. Int J Syst Evol Microbiol, January 1, 2005; 55(1): 449 - 456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, M. Bisgaard, O. Angen, W. Frederiksen, and J. E. Olsen Characterization of Sucrose-Negative Pasteurella multocida Variants, Including Isolates from Large-Cat Bite Wounds J. Clin. Microbiol., January 1, 2005; 43(1): 259 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kuhnert, B. Korczak, E. Falsen, R. Straub, A. Hoops, P. Boerlin, J. Frey, and R. Mutters Nicoletella semolina gen. nov., sp. nov., a New Member of Pasteurellaceae Isolated from Horses with Airway Disease J. Clin. Microbiol., December 1, 2004; 42(12): 5542 - 5548. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, P. Kuhnert, J. E. Olsen, and M. Bisgaard Comparative phylogenies of the housekeeping genes atpD, infB and rpoB and the 16S rRNA gene within the Pasteurellaceae Int J Syst Evol Microbiol, September 1, 2004; 54(5): 1601 - 1609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Davies and I. Lee Sequence Diversity and Molecular Evolution of the Heat-Modifiable Outer Membrane Protein Gene (ompA) of Mannheimia(Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi J. Bacteriol., September 1, 2004; 186(17): 5741 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Korczak, H. Christensen, S. Emler, J. Frey, and P. Kuhnert Phylogeny of the family Pasteurellaceae based on rpoB sequences Int J Syst Evol Microbiol, July 1, 2004; 54(4): 1393 - 1399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, M. Bisgaard, B. Aalbaek, and J. E. Olsen Reclassification of Bisgaard taxon 33, with proposal of Volucribacter psittacicida gen. nov., sp. nov. and Volucribacter amazonae sp. nov. as new members of the Pasteurellaceae Int J Syst Evol Microbiol, May 1, 2004; 54(3): 813 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Esteve, L. Valera, C. Gutierrez, and A. Ventosa Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992 Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1411 - 1419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Angen, P. Ahrens, P. Kuhnert, H. Christensen, and R. Mutters Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis' Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1449 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Christensen, M. Bisgaard, A. M. Bojesen, R. Mutters, and J. E. Olsen Genetic relationships among avian isolates classified as Pasteurella haemolytica, 'Actinobacillus salpingitidis' or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov. Int J Syst Evol Microbiol, January 1, 2003; 53(1): 275 - 287. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Davies, S. Campbell, and T. S. Whittam Mosaic Structure and Molecular Evolution of the Leukotoxin Operon (lktCABD) in Mannheimia (Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi J. Bacteriol., January 1, 2002; 184(1): 266 - 277. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kehrenberg, S. A. Salmon, J. L. Watts, and S. Schwarz Tetracycline resistance genes in isolates of Pasteurella multocida, Mannheimia haemolytica, Mannheimia glucosida and Mannheimia varigena from bovine and swine respiratory disease: intergeneric spread of the tet(H) plasmid pMHT1 J. Antimicrob. Chemother., November 1, 2001; 48(5): 631 - 640. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kehrenberg and S. Schwarz Molecular Analysis of Tetracycline Resistance in Pasteurella aerogenes Antimicrob. Agents Chemother., October 1, 2001; 45(10): 2885 - 2890. [Abstract] [Full Text]
|
|
|
|
|
|
P. G. Mbuthia, H. Christensen, M. Boye, K. M. D. Petersen, M. Bisgaard, P. N. Nyaga, and J. E. Olsen Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization J. Clin. Microbiol., July 1, 2001; 39(7): 2627 - 2633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Davies, T. S. Whittam, and R. K. Selander Sequence Diversity and Molecular Evolution of the Leukotoxin (lktA) Gene in Bovine and Ovine Strains of Mannheimia (Pasteurella) haemolytica J. Bacteriol., February 15, 2001; 183(4): 1394 - 1404. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
