| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Centro de Investigacién sobre Fijactón de Nitrógeno, UNAM, Apdo Postal 565-A, Cuernavaca, Morelos, Mexico
2Alfalfa and Soybean Research Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA
3Department of Microbiology, College of Biology, China Agricultural University, Beijing 100094, People's Republic of China
Author for correspondence: E. T. Wang. Tel: +52 73 131697. Fax: +52 73 175581. e-mail: ewang{at}cifn.unam.mx
ABSTRACT
Fifty-five Chinese isolates from nodules of Amorpha fruticosa were characterized and compared with the type strains of the species and genera of bacteria which form nitrogen-fixing symbioses with leguminous host plants. A polyphasic approach, which included RFLP of PCR-amplified 16S rRNA genes, multilocus enzyme electrophoresis (MLEE), DNA-DNA hybridization, 16S rRNA gene sequencing, electrophoretic plasmid profiles, cross-nodulation and a phenotypic study, was used in the comparative analysis. The isolates originated from several different sites in China and they varied in their phenotypic and genetic characteristics. The majority of the isolates had moderate to slow growth rates, produced acid on YMA and harboured a 930 kb symbiotic plasmid (pSym). Five different RFLP patterns were identified among the 16S rRNA genes of all the isolates. Isolates grouped by PCR-RFLP of the 16S rRNA genes were also separated into groups by variation in MLEE profiles and by DNA-DNA hybridization. A representative isolate from each of these DNA homology groups had a separate position in a phylogenetic tree as determine from sequencing analysis of the 16S rRNA genes. A new species, Mesorhizobium amorphae, is proposed for the majority of the isolates, which belonged to a moderately slow- to slow-growing, acid-producing group based upon their distinct phylogenetic position, their unique electrophoretic type, their low DNA homology with reference strains representing the species within the genus Mesorhizobium and their distinct phenotypic features. Strain ACCC 19665 was chosen as the type strain for M. amorphae sp. nov.
Key Words: Mesorhizobium amorphae • Amorpha fruticosa • polyphasic taxonomy • diversity • rhizobia
The GenBank accession numbers for the 16S rRNA gene sequences of isolates ACCC 19665T, ACCC 19667, H15003, HL56 and SH283012, and of M. tianshanense A-1BSTare AF041442--AF041447, respectively.
This article has been cited by other articles:
|
|
 |
|
  F. Q. Wang, E. T. Wang, J. Liu, Q. Chen, X. H. Sui, W. F. Chen, and W. X. Chen Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1192 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Hunter, H. J. Mills, and J. E. Kostka Microbial Community Diversity Associated with Carbon and Nitrogen Cycling in Permeable Shelf Sediments Appl. Envir. Microbiol., September 1, 2006; 72(9): 5689 - 5701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ghosh and P. Roy Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant Int J Syst Evol Microbiol, January 1, 2006; 56(1): 91 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Germano, P. Menna, F. L. Mostasso, and M. Hungria RFLP analysis of the rRNA operon of a Brazilian collection of bradyrhizobial strains from 33 legume species Int J Syst Evol Microbiol, January 1, 2006; 56(1): 217 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Gao, S. L. Turner, F. L. Kan, E. T. Wang, Z. Y. Tan, Y. H. Qiu, J. Gu, Z. Terefework, J. P. W. Young, K. Lindstrom, et al. Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2003 - 2012. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Wei, Z. Y. Tan, M. E Zhu, E. T. Wang, S. Z. Han, and W. X. Chen Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov. Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1575 - 1583. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. L. Simms and D. L. Taylor Partner Choice in Nitrogen-Fixation Mutualisms of Legumes and Rhizobia Integr. Comp. Biol., April 1, 2002; 42(2): 369 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Parker Case of Localized Recombination in 23S rRNA Genes from Divergent Bradyrhizobium Lineages Associated with Neotropical Legumes Appl. Envir. Microbiol., May 1, 2001; 67(5): 2076 - 2082. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Laguerre, S. M. Nour, V. Macheret, J. Sanjuan, P. Drouin, and N. Amarger Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts Microbiology, April 1, 2001; 147(4): 981 - 993. [Abstract] [Full Text]
|
|
|
|
|
|
A. Ulrich and I. Zaspel Phylogenetic diversity of rhizobial strains nodulating Robinia pseudoacacia L. Microbiology, November 1, 2000; 146(11): 2997 - 3005. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Parker Relationships of Bradyrhizobia from the Legumes Apios americana and Desmodium glutinosum Appl. Envir. Microbiol., November 1, 1999; 65(11): 4914 - 4920. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
