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Zygosaccharomyces lentus sp. nov., a new member of the yeast genus Zygosaccharomyces Barker

Hazel Steels¹, Christopher J. Bond², Matthew D. Collins^3,, Ian N. Roberts², Malcolm Stratford¹ and Stephen A. James²

¹Microbiology Section, Unilever Research, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK
²National Collection of Yeast Cultures, Department of Genetics and Microbiology, Institute of Food Research, Norwich Laboratory, Norwich NR4 7UA, UK
³Department of Microbiology, Institute of Food Research, Reading Laboratory, Reading RG6 6BZ, UK

Author for correspondence: Stephen A. James. Tel: +44 1603 255274. Fax: +44 1603 458414. e-mail: Steve.James{at}bbsrc.ac.uk

ABSTRACT

Unusual growth characteristics of a spoilage yeast, originally isolated from spoiled whole-orange drink and previously identified as Zygosaccharomyces bailii, prompted careful re-examination of its taxonomic position. Smallsubunit rRNA gene sequences were determined for this strain and for four other strains also originally described as Z. bailii but which, in contrast to other strains of this species, grew poorly or not at all under aerobic conditior with agitation, failed to grew in the presence of 1% acetic acid and failed to grow at 30 °C. Comparative sequence analysis revealed that these strains represented a phylogenetically distinct taxon closely related to, but distinc from, Z. bailii and Zygosaccharomyces bisporus. Furthermore, sequence analysis of the internal transcribed spacer (ITS) region showed that, while all five strains had identical ITS2 sequences, they could be subdivided into two groups based on ITS1 sequences. Despite such minor inter-strain sequence variation, these yeasts could readily be distinguished from all other currently described Zygosaccharomyces species by using ITS sequences. On the basis of the phylogenetic results presented, a new species comprising the five strains Zygosaccharomyces lentus sp. nov., is described and supporting physiological data are discussed, including a demonstration that growth of this species is particularly sensitive to the presence of oxygen. The type strain of Z. lentus is NCYC D2627^T.

Key Words: yeast • Zygosaccharomyces lentus • preservative resistance • spoilage

The EMBL accession numbers for the sequences reported in this paper are Y16814 (18S rDNA of NCYC D2627^T), Y16815 (ITS1 region of NCYC D2627^T), Y16816 (ITS2 region of NCYC D2627^T) and Y16817 (ITS1 region of NCYC 2406).

Present address: Dept of Food Science and Technology, Earley Gate, University of Reading, Reading RG6 6BZ, UK.