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Int J Syst Bacteriol 49 (1999), 267-275; DOI 10.1099/00207713-49-1-267
© 1999 Society for General Microbiology
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Sodalis gen. nov. and Sodalis glossinidius sp. nov., a microaerophilic secondary endosymbiont of the tsetse fly Glossina morsitans morsitans

Colin Dale{dagger} and Ian Maudlin

Division of Molecular Genetics, University of Glasgow, Glasgow G11 6NU, UK

Author for correspondence: Colin Dale. Tel: +44 1904 434383. Fax: +44 1904 434383. e-mail: cdl5{at}york.ac.uk

ABSTRACT

A secondary intracellular symbiotic bacterium was isolated from the haemolymph of the tsetse fly Glossina morsitans morsitans and cultured in Aedes albopictus cell line C6/36. Pure-culture isolation of this bacterium was achieved through the use of solid-phase culture under a microaerobic atmosphere. After isolation of strain M1T, a range of tests was performed to determine the phenotypic properties of this bacterium. Considering the results of these tests, along with the phylogenetic position of this micro-organism, it is proposed that this intracellular symbiont from G. m. morsitans should be classified in a new genus Sodalis gen. nov., as Sodalis glossinidius gen. nov., sp. nov. Strain M1Tis the type strain for this new species.


Key Words: Sodalis glossinidius gen. nov. • sp. nov. • insect endosymbiont • tsetse • microaerophile

{dagger} Present address: Department of Biology, University of York, Heslington, York YO1 5YW, UK.




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