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1Department of Applied Chemistry and Microbiology, PO Box 56 (Biocentre), 00014 University of Helsinki, Finland
2Department of Microbiology, 508 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA
3DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen, D-38124 Braunschweig, Germany
4Institut fuer Angewandte Mikrobiologie, Justus-Liebig Universität, Senckenbergstr. 3, D-35390 Giessen, Germany
5National Public Health Institute, Department in Turku, Kiinamyllynkatu 13, FIN-20520 Turku, Finland
Author for correspondence: R. Vuorio. Tel: +358 9 708 59333. Fax: +358 9 708 59322. e-mail: Riitta.Vuorio{at}Helsinki.fi
ABSTRACT
Scotochromogenic mycobacterial isolates from water-damaged parts of indoor building materials of a children's day care centre represented a phenetically and genetically distinct group of strains. A 16S rDNA dendrogram (1243 bp) showed that the closest species to the new strain MA112/96Twas Mycobacterium abscessus. Phylogenetic and phenetic analyses (100 characteristics) grouped the new isolates with M. abscessus, Mycobacterium vaccae, Mycobacterium aurum and Mycobacterium austroafricanum. Ribotyping with Pvull restriction distinguished the 5 isolates from the other 12 most closely related species by the major bands at 6·5--7 kb and 13--15 kb. The cell morphology of the new isolates was typical of mycobacteria, electron microscopy revealed a triple-layered cell wall with an irregular electron-dense outer layer. They grew at 10--37 °C with no growth at 45 °C in 5 d. The gene encoding the secreted 32 kDa protein, specific to mycobacteria, was detected by PCR. The main whole-cell fatty acids were characterized by high tuberculostearic acid 10Me-C18:0 (17% at 28 °C), which increased with increasing growth temperature (22% at 37 °C). The other main fatty acids were C18:1 cis9 and C16:0 (21--20% each), followed by, C17:1 cis9 (14%), C16:1 cis10 (8%) and also a high amount of C20 alcohol (9%). 
-Mycolic acids, keto-mycolates and wax esters were present (C60-C90), MK-9(H2) (90%) and MK-8(H2) were the main menaquinones. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidyl inositolmannosides and diphosphatidylglycerol. Polyamine content was low. G+C content was 72·9 mol%. The new isolates are proposed as a new species, Mycobacterium murale sp. nov. The type strain is MA112/96T(= DSM 44340T).
Key Words: indoor contaminant • ribotyping • 16S rDNA • Mycobacterium abscessus • Mycobacterium komossense
Present address: Institut fuer Bakteriologie und Tierhygiene, Veterinärmedizinische Universität, Josef Baumann Gasse 1, A-1210 Wien, Austria.
The GenBank accession number for the 16S rDNA sequence of strain MA1 12/96T(= DSM 44340T) is Y08857.
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