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Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen

¹MBI International, 3900 Collins Road, PO Box 27609, Lansing, Ml 48909, USA
²Department of Animal Science, Michigan State University, East Lansing, Ml 48824, USA

Author for correspondence: Mahendra K. Jain (MBI International). Tel: +1 517 336 4626. Fax: +1 517 337 2122. e-mail: jain{at}mbi.org

ABSTRACT

Strain 130Z^Twas isolated from the bovine rumen. It is a facultatively anaerobic, pleomorphic, Gram-negative rod. It exhibits a ‘Morse code’ form of morphology, which is characteristic of the genus Actinobacillus. Strain 130Z^Tis a capnophilic, osmotolerant succinogen that utilizes a broad range of sugars. It accumulates high concentrations of succinic acid (> 70gl^-1). Strain 130Z^Tis positive for catalase, oxidase, alkaline phosphatase and β-galactosidase, but does not produce indole or urease. Acid but no gas is produced from D-glucose and D-fructose. 16S rRNA sequence analysis places strain 130Z^Twithin the family Pasteurellaceae; the most closely related members of the family Pasteurellaceae have 16S rRNA similarities of 95.5% or less with strain 130Z^T. Strain 130Z^Twas compared with Actinobacillus lignieresii and the related Bisgaard Taxa 6 and 10. Based upon morphological and biochemical properties, strain 130Z^Tis most similar to members of the genus Actinobacillus within the family Pasteurellaceae. It is proposed that strain 130Z^Tbe classified as a new species, Actinobacillus succinogenes. The type strain of Actinobacillus succinogenes sp. nov. is ATCC 55618^T.

Key Words: Actinobacillus succinogenes sp. nov. • succinic acid • rumen • Pasteurellaceae

The GenBank accession number for the 16S rRNA sequence of strain 130Z^Tis AF024525.

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