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1Institute of Medical Microbiology and Hygiene, University of Regensburg, Franz-Josef-StrauøZ-Allee 11, D-93053 Regensburg, Germany
2Central Laboratory of Bacteriology, University Hospital Geneva, 1211 Geneva, Switzerland
3Masaryk Hospital, Usti N. Labem, Czech Republic
4National Reference Center for Mycobacterium kansasii, 72892 Ostrava, Czech Republic
5Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, D-38124. Braunschweig, Germany
6Tel: +49 941 59609 50. Fax: +49 941 944 6402. e-mail: Udo.Reischl{at}klinik.uni-regensburg.de
ABSTRACT
A new, slow-growing, scotochromogenic mycobacterium was isolated from sputum of a 53-year-old patient with Down's syndrome suffering from tuberculosis. Growth occurred at temperatures between 25 and 40°C with an optimum at 37°C. This strain had surprisingly few enzymic activities (only positive for 68°C heat-stable catalase and weakly positive for urease) and was sensitive to prothionamide, cycloserine, clarithromycin, gentamicin and amikacin but showed resistance to isoniazid, streptomycin, ethambutol, rifampin and ciprofloxacin. These characteristics assign this organism to a novel mycobacterial species characterized by a unique 16S rDNA nucleotide sequence. The name Mycobacterium bohemicum sp. nov. is proposed for this new, slow-growing, scotochromogenic mycobacterium. The type strain is DSM 44277T.
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