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Application of the Ribonuclease P (RNase P) RNA Gene Sequence for Phylogenetic Analysis of the Genus Saccharomonospora

¹Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305-600, Korea

²Tel: +82 42 860 4620. Fax: +82 42 860 4625. e-mail: yhpark{at}kribb4680.kribb.re.kr

ABSTRACT

Sequences of the RNase P RNA gene were investigated for the phylogenetic analysis of the genus Saccharomonospora. Aligned nucleotide sequences, determined from the PCR-amplified RNase P RNA gene of representative strains of the genus Saccharomonospora, displayed 94·2±1·3% interspecific variances. The intraspecific similarity value was 99·7–100% in all species tested. Saccharomonospora azurea K161^Tand ‘Saccharomonospora caesia’ K76^Tdisplayed identical RNase P RNA gene sequences in the region that was determined and Saccharomonospora sp. K180 showed sequences distinct from validly described species with a similarity value of 94·6±10%. The phylogenetic trees constructed by aligning the sequences either within the genus Saccharomonospora or with other Gram-positive bacteria were similar to the ones derived using sequences of the 16S rDNA gene. Advantageous features of this gene for application as a molecular phyletic marker are discussed.