| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1North Carolina State University, Raleigh, NC 27695–7614, USA
2Dade MicroScan Inc., West Sacramento, CA 95691, USA
3Framingham State College, Framingham, MA 01701, USA
4Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, New Brunswick, NJ 08903–0019, USA
5Duke University Medical Center, Durham, NC 27710, USA
Author for correspondence: Wesley E. Kloos. Tel: +1 919 515 5781. Fax: +1 919 515 3355. e-mail: wesley_kloos{at}ncsu.edu
ABSTRACT
A new subspecies, Staphylococcus hominis subsp. novobiosepticus, isolated from human blood cultures, a wound, a breast abscess and a catheter tip, is described on the basis of a study of 26 strains isolated between 1989 and 1996. DNA-DNA reassociation reactions, conducted under stringent conditions, and macrorestriction pattern analysis demonstrated that these strains are closely related to previously characterized S. hominis strains isolated from human skin and clinical specimens, but are significantly divergent. S. hominis subsp. novobiosepticus can be distinguished from S. hominis (now named S. hominis subsp. hominis) by its combined characteristics of novobiocin resistance and failure to produce acid aerobically from D-trehalose and N-acetyl-D-glucosamine. Furthermore, all 26 strains of the new subspecies are resistant to nalidixic acid, penicillin G, oxacillin, kanamycin and streptomycin, and were either resistant or had intermediate resistance to methicillin and gentamicin. Most strains were also resistant to erythromycin, clindamycin, chloramphenicol, trimethoprim/sulfamethoxazole and ciprofloxacin. Based on a comparison of the sequences of a 1001 bp mecA amplification product from reference methicillin-resistant staphylococci, the mecA gene present in S. hominis subsp. novobiosepticus was identified as homologue A, commonly found in S. aureus and many coagulase-negative staphylococcal species. The type strain of S. hominis subsp. novobiosepticus is ATCC 700236T. Descriptions of S. hominis subsp. novobiosepticus subsp. nov. and S. hominis subsp. hominis are given and the description of S. hominis is emended.
Key Words: Staphylococcus hominis subsp. novobiosepticus subsp. nov. • Staphylococcus hominis subsp. hominis • multiple antibiotic resistance • mecA gene homologues • novobiocin-resistant Staphylococcus hominis
This article has been cited by other articles:
|
|
 |
|
  I. C. V. Palazzo, P. A. d'Azevedo, C. Secchi, A. C. C. Pignatari, and A. L. d. C. Darini Staphylococcus hominis subsp. novobiosepticus strains causing nosocomial bloodstream infection in Brazil J. Antimicrob. Chemother., December 1, 2008; 62(6): 1222 - 1226. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Brigante, M. G. Menozzi, B. Pini, R. Porta, P. Somenzi, A. Sciacca, T. Spanu, and S. Stefani Identification of Coagulase-Negative Staphylococci by Using the BD Phoenix System in the Low-Inoculum Mode J. Clin. Microbiol., November 1, 2008; 46(11): 3826 - 3828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. d'Azevedo, R. Trancesi, T. Sales, J. Monteiro, A. C. Gales, and A. C. Pignatari Outbreak of Staphylococcus hominis subsp. novobiosepticus bloodstream infections in Sao Paulo city, Brazil J. Med. Microbiol., February 1, 2008; 57(2): 256 - 257. [Full Text] [PDF]
|
|
|
|
|
|
J. Gilad, D. Schwartz, F. Layer, B. Ghebremedhin, K. A. Moder, W. Konig, and B. Konig Identification of Staphylococcus Species with the VITEK 2 System: the Case of Staphylococcus hominis J. Clin. Microbiol., February 1, 2007; 45(2): 685 - 686. [Full Text] [PDF]
|
|
|
|
|
|
F. Chaves, M. Garcia-Alvarez, F. Sanz, C. Alba, and J. R. Otero Nosocomial Spread of a Staphylococcus hominis subsp. novobiosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit J. Clin. Microbiol., September 1, 2005; 43(9): 4877 - 4879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Spanu, M. Sanguinetti, D. Ciccaglione, T. D'Inzeo, L. Romano, F. Leone, and G. Fadda Use of the VITEK 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections J. Clin. Microbiol., September 1, 2003; 41(9): 4259 - 4263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. T. Dubin, J. E. Fitzgibbon, M. D. Nahvi, and J. F. John Topoisomerase Sequences of Coagulase-Negative Staphylococcal Isolates Resistant to Ciprofloxacin or Trovafloxacin Antimicrob. Agents Chemother., July 1, 1999; 43(7): 1631 - 1637. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
