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Rhizobium huautlense sp. nov., a symbiont of Sesbania herbacea that has a close phylogenetic relationship with Rhizobium galegae

¹Departamento de Genética Molecular, Centro de Investigación sobre Fijación de Nitrógeno, Apdo Postal 565-A, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
²Soybean and Alfalfa Research Laboratory, Agricutural Research Service, US Department of Agriculture, Beltsville, MD 20705, USA
³College of Agriculture and Natural Resources, The Department of Natural Resource Sciences and Landscape Architecture, University of Maryland, College Park, MD 20742, USA
⁴Department of Microbiology, College of Biology, Beijing Agricultural University, Beijing 100094, People's Republic of China
⁵CIEH, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México

Author for correspondence: E. Martínez-Romero. Tel: +52 73 13 16 97. Fax: +52 73 17 55 81. e-mail: emartine{at}cifn.unam.mx

ABSTRACT

The nitrogen-fixing rhizobial symbionts of Sesbania herbacea growing in the nature reserve at the Sierra de Huautla, Mexico, were isolated and characterized. All 104 isolates together with the type strain for Rhizobium galegae, HAMBI 540^T, had similar 16S rRNA genes as revealed by PCR-RFLP analysis. Similarity in the sequences of the 16S rRNA genes placed the isolates on a phylogenetic branch shared with R. galegae. Among 66 randomly selected isolates, three closely related electrophoretic alloenzyme types (ETs) were identified, which were distinct from 10 ETs distinguished among 23 strains of R. galegae. A new species Rhizobium huautlense, represented by the Sesbania isolate S02^T, is proposed based upon low estimates of DNA relatedness between our chosen type strain and the type strains for the other species, the dissimilarity of the nucleotide sequence of the 16S rRNA genes, and their distinct ETs compared with R. galegae. The description of R. huautlense is significant because in the reconstruction of the phylogeny of R. huautlense there was a shift in the node of the branch of Agrobacterium vitis relative to that of R. galegae. The revised phylogenetic tree would tend to indicate common ancestry between R. galegae and Rhizobium leguminosarum.

Key Words: Rhizobium huautlense sp. nov. • symbionts • Sesbania herbacea • nitrogen fixers

The GenBank accession numbers for the 16S rRNA gene sequences of strains 502^Tand 59A2 are AF025852 and AF025853, respectively.

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