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DNA sequencing reveals limited heterogeneity in the 16S rRNA gene from the rrnB operon among five Mycoplasma hominis isolates

Department of Medical Microbiology and Immunology, The Bartholin Building, University of Aarhus, DK-8000 Aarhus C, Denmark

Author for correspondence: Gunna Christiansen. Tel: +45 8942 1749. Fax: +45 8619 6128. e-mail: chlam{at}biobase.dk

ABSTRACT

To investigate the intraspecies heterogeneity within the 16S rRNA gene of Mycoplasma hominis, five isolates with diverse antigenic profiles, variable/identical P120 hypervariable domains, and different 16S rRNA gene RFLP patterns were analysed. The 16S rRNA gene from the rrnB operon was amplified by PCR and the PCR products were sequenced. Three isolates had identical 16S rRNA sequences and two isolates had sequences that differed from the others by only one nucleotide.

Key Words: Mycoplasma hominis • 16S rRNA • variation • RFLP analysis • intraspecies heterogeneity

The EMBL accession numbers for the 16S rRNA gene sequences from the rrnB operon of M. hominis strains reported in this paper are as follows: strain PG21^T, AJ002265; strain DC63, AJ002266; strain SC4, AJ002267; strain P2, AJ002268; strain 7488, AJ002269; and strain 183, AJ002270.

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