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Petrotoga mobilis sp. nov., from a North Sea oil-production well

Torleiv Lien¹, Marit Madsen¹, Fred A. Rainey^2, and Nils-Kåre Birkeland¹

¹Department of Microbiology, University of Bergen, IM, Jahnebakken 5, N-5020 Bergen, Norway
²DSMZ, Mascheroder Weg 1b, D-38124 Braunschweig, Germany

Author for correspondence: Torleiv Lien. Tel: +47 55582669. Fax: +47 55589671. e-mail: torleiv.lien{at}im.uib.no

Author for correspondence: Torleiv Lien. Tel: +47 55582669. Fax: +47 55589671. e-mail: torleiv.lien{at}im.uib.no

ABSTRACT

Rod-shaped, thermophilic bacteria with a sheath-like outer structure (‘toga’) were isolated from hot oilfield water of a North Sea oil reservoir. One of the isolates, designated SJ95^T, is an obligately anaerobic, sheathed, Gram-negative, fermentative bacterium capable of reducing elemental sulfur to hydrogen sulfide and tolerating high salt concentrations. The optimum growth conditions for this isolate are 58–60°C and pH 6·5–7·0 with 3–4% NaCl and 0·7% MgSO₄ .7H₂O in the medium. Vitamins are required for growth. Growth is stimulated by yeast extract. Cells of strain SJ95^Tvary in size from 1–2 to 40–50 µm in length and are motile with a subpolar flagellation. Cells grown on xylan have xylanase activity, presumably associated with the toga, and glucose isomerase activity was detected in xylose-grown cells. The DNA G+C content is 31 and 34 mol%, determined by the thermal denaturation and HPLC methods, respectively. Phylogenetically, strain SJ95^Tis most closely related to Petrotoga miotherma with a 97·7% similarity level between their 16S rDNA sequences. The DNA-DNA reassociation value between the two DNAs was 35·6%. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain SJ95^T(= DSM 10674^T) is proposed as the type strain of a new species, Petrotoga mobilis. It can be readily distinguished from P. miotherma by its motility.

Key Words: Petrotoga mobilis sp. nov. • thermophile • oilfield water

The EMBL accession number for the 16S rDNA sequence of strain SJ95^Treported in this paper is Y15479.

Department of Biological Sciences, 508 Life Sciences Building, Louisiana State University, Baton Rouge, LA 70803, USA.

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