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1Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, CP 5000, St-Hyacinthe, Québec, Canada
2Biotechnology Research Institute, Montreal, Canada
3Faculty of Veterinary Medicine, University of Ghent, Merelbeke, Belgium
Author for correspondence: Josée Harel. Tel: +1 514 773 8521, ext. 8233. Fax: + 1 514 778 8108. e-mail: HarelJ{at}ere.umontreal.ca
ABSTRACT
The 16S rRNA gene sequences of reference strains of Streptococcus suis serotypes 1–34 and 1/2 were determined. A comparative sequence analysis showed that the degree of sequence similarity between S. suis reference strains ranged from 93·94 to 100%. A dendrogram was constructed from the similarity matrix. Thirty-two strains representing 32 serotypes fell into a major group divided into three clusters. The other strains, S. suis serotypes 32, 33 and 34, were more distant. Biochemical characterization of the six more distant strains, including S. suis serotypes 20, 22, 26, 32, 33 and 34, revealed a profile similar to that of other S. suis serotypes. Comprison of the 16S rRNA gene sequences of S. suis reference strains with sequences of other members of the genus Streptococcus indicated that, with the exception of S. suis serotypes 32, 33 and 34, reference strains did not cluster with any other species in the genus. In conclusion, 16S rRNA gene sequence analysis defined a major group of S. suis reference strains which were very closely related and a higher divergence for S. suis serotypes 32, 33 and 34. However, to date, there is no strong evidence to reclassify strains of these serotypes in another species.
Key Words: Streptococcus suis • 16S rRNA gene sequence analysis
The GenBank accession numbers for the 16S rRNA gene sequences of S. suis determined in this study are AF009475–AF009509.
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