HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sawabe, T. Articles by Christen, R. Search for Related Content PubMed Articles by Sawabe, T. Articles by Christen, R. Agricola Articles by Sawabe, T. Articles by Christen, R.

Vibrio halioticoli sp. nov., a non-motile alginolytic marine bacterium isolated from the gut of the abalone Haliotis discus hannai

Tomoo Sawabe^1,, Itsuro Sugimura¹, Miwa Ohtsuka¹, Kazuaki Nakano¹, Kenichi Tajima¹, Yoshio Ezura¹ and Richard Christen²

¹Laboratory of Microbiology, Faculty of Fisheries, Hokkaido University, 3-1-1 Minatocho, Hakodate 041, Japan
²Centre National de la Recherche Scientifique et Université, Pierre et Marie Curie, Station Zoologique, Villefranche-sur-Mer 06230, France

Author for correspondence: Tomoo Sawabe. Tel/Fax: +81 138 40 5569. e-mail: sawabe{at}pop.fish.hokudai.ac.jp

ABSTRACT

Six alginolytic, facultatively anaerobic, non-motile marine bacteria were isolated from the gut of abalone Haliotis discus hannai. DNA-DNA hybridization data showed that the six strains constituted a single genospecies. Phylogenetic analyses of 16S rDNA sequences indicated that the isolates should be assigned to the genus Vibrio. The phenotypic features of the isolates were closely related to Vibrio fischeri and Vibrio pelagius biovar I, but 13 traits (motility, luminescence, alginase production, lipase production, lysine decarboxylase, indole production, growth in 1 and 6% NaCl and assimilation of five carbon compounds) distinguished these strains from V. fischeri, and 17 traits (motility, growth at 37 °C, lipase production, indole production, growth in 1 and 6% NaCl, acid from sucrose and D-sorbitol, and assimilation of nine carbon compounds) distinguished these strains from V. pelagius. The G+C content of the isolates was 41·6–43·1 mol%. According to DNA-DNA hybridization data and 16S rDNA phylogenetic analyses, it was concluded that the six isolates constitute a new species different from any other Vibrio species. The name Vibrio halioticoli sp. nov. (type strain IAM 14596^T) is proposed. A set of phenotypic features which enables differentiation of the new species from other species of the Vibrionaceae family is described.

Key Words: Vibrio halioticoli sp. nov. • marine bacterium • alginolytic • gut microflora • abalone

The DDBJ/EMBL/GenBank accession numbers for the nucleotide sequences of V. halioticoli are AB000390 (lAM 14596^T= A431), AB000391 (lAM 14597 = A611), AB000392 (lAM 14598 = A1013) and AB000393 (lAM 14599 = A1112).

Present address: Dept of Biological Sciences, The Manchester Metropolitan University, John Dalton Building, Chester Street, Manchester M1 5GD, UK. Tel: +44 161 247 1157; Fax: +44 161 247 6365; e-mail: t.sawabe@mmu.ac.uk/ (until August 1998).

This article has been cited by other articles:

				N. Rameshkumar, Y. Fukui, T. Sawabe, and S. Nair Vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (Porteresia coarctata Tateoka) Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1608 - 1615. [Abstract] [Full Text] [PDF]

				T. Sawabe, Y. Fujimura, K. Niwa, and H. Aono Vibrio comitans sp. nov., Vibrio rarus sp. nov. and Vibrio inusitatus sp. nov., from the gut of the abalones Haliotis discus discus, H. gigantea, H. madaka and H. rufescens Int J Syst Evol Microbiol, May 1, 2007; 57(5): 916 - 922. [Abstract] [Full Text] [PDF]

				T. Sawabe, K. Hayashi, J. Moriwaki, F. L. Thompson, J. Swings, P. Potin, R. Christen, and Y. Ezura Vibrio gallicus sp. nov., isolated from the gut of the French abalone Haliotis tuberculata Int J Syst Evol Microbiol, May 1, 2004; 54(3): 843 - 846. [Abstract] [Full Text] [PDF]

				E. P. Ivanova, N. M. Gorshkova, T. Sawabe, N. V. Zhukova, K. Hayashi, V. V. Kurilenko, Y. Alexeeva, V. Buljan, D. V. Nicolau, V. V. Mikhailov, et al. Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina) Int J Syst Evol Microbiol, March 1, 2004; 54(2): 475 - 480. [Abstract] [Full Text] [PDF]

				K. Hayashi, J. Moriwaki, T. Sawabe, F. L. Thompson, J. Swings, N. Gudkovs, R. Christen, and Y. Ezura Vibrio superstes sp. nov., isolated from the gut of Australian abalones Haliotis laevigata and Haliotis rubra Int J Syst Evol Microbiol, November 1, 2003; 53(6): 1813 - 1817. [Abstract] [Full Text] [PDF]

				E. P. Ivanova, T. Sawabe, K. Hayashi, N. M. Gorshkova, N. V. Zhukova, O. I. Nedashkovskaya, V. V. Mikhailov, D. V. Nicolau, and R. Christen Shewanella fidelis sp. nov., isolated from sediments and sea water Int J Syst Evol Microbiol, March 1, 2003; 53(2): 577 - 582. [Abstract] [Full Text] [PDF]

				T. Sawabe, F. L. Thompson, J. Heyrman, M. Cnockaert, K. Hayashi, R. Tanaka, M. Yoshimizu, B. Hoste, J. Swings, and Y. Ezura Fluorescent Amplified Fragment Length Polymorphism and Repetitive Extragenic Palindrome-PCR Fingerprinting Reveal Host-Specific Genetic Diversity of Vibrio halioticoli-Like Strains Isolated from the Gut of Japanese Abalone Appl. Envir. Microbiol., August 1, 2002; 68(8): 4140 - 4144. [Abstract] [Full Text] [PDF]

				I. Yumoto, H. Iwata, T. Sawabe, K. Ueno, N. Ichise, H. Matsuyama, H. Okuyama, and K. Kawasaki Characterization of a Facultatively Psychrophilic Bacterium, Vibrio rumoiensis sp. nov., That Exhibits High Catalase Activity Appl. Envir. Microbiol., January 1, 1999; 65(1): 67 - 72. [Abstract] [Full Text]