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Bacillus horti sp. nov., a new Gram-negative alkaliphilic bacillus

¹Bioscience and Chemistry Division, Hokkaido National Industrial Research Institute, 2-17-2-1 Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan
²Department of Marine Bioresources Chemistry, Faculty of Fisheries, Hokkaido University, Hakodate 041-0821, Japan
³Department of Fisheries Oceanography & Marine Science, Faculty of Fisheries, Hokkaido University, Hakodate 041-0821, Japan

Author for correspondence: Isao Yumoto. Tel: +81 11 857 8925. Fax: +81 11 857 8900. e-mail: yumoto{at}hniri.go.jp

ABSTRACT

Novel Gram-negative alkaliphilic strains were isolated from soil obtained from Atsuma, Hokkaido, Japan. The isolates were strictly aerobic rods that produced subterminally located ellipsoidal spores. Chemotaxonomic characteristics of the isolates included the presence of meso-diaminopimelic acid in the cell wall and a DNA G+C content of 40·2–40·9 mol%. The major isoprenoid quinone was menaquinone-7 and the cellular fatty acid profile consisted of a significant amount of 15-C branched-chain acids, iso-C_15:0 and anteiso-C_15:0. The growth rate was higher at pH 8–10 than at pH 7. Comparative sequence analysis of 16S rDNA of 14 alkaliphilic Bacillus strains indicates that the isolated strain has an equidistant relationship to three already defined rRNA groups of alkaliphilic Bacillus species. Based on the morphological and physiological characteristics, as well as phylogenetic position as determined by 16S rDNA analysis and DNA-DNA relatedness data, it is concluded that these isolates should be designated as a new species, for which the name Bacillus horti is proposed. The type strain is K13^T(= JCM 9943^T).

Key Words: Bacillus horti sp. nov. • alkaliphilic • Gram-negative bacillus chemotaxonomic analysis • 16S rDNA analysis

The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence reported in this paper is D87035.

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