HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Miroshnichenko, M. L. Articles by Bonch-Osmolovskaya, E. A. Search for Related Content PubMed Articles by Miroshnichenko, M. L. Articles by Bonch-Osmolovskaya, E. A. Agricola Articles by Miroshnichenko, M. L. Articles by Bonch-Osmolovskaya, E. A.

Desulfurella kamchatkensis sp. nov. and Desulfurella propionica sp. nov., new sulfur-respiring thermophilic bacteria from Kamchatka thermal environments

¹Institute of Microbiology, Russian Academy of Sciences, Prospect 60 Letiya Oktyabrya 7/2, 117811 Moscow, Russia
²German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

Author for correspondence: M. L. Miroshnichenko. Tel: +7 95 1350131. Fax: +7 95 1356530. e-mail: mir{at}imbran.msk.su

ABSTRACT

Two strains of moderately thermophilic bacteria, which reduce elemental sulfur to hydrogen sulfide, were isolated from volcanic sources in Kamchatka. Strain K-119^Twas obtained from a thermophilic microbial community associated with Thermothrix thiopara, and strain U–8^Twas isolated from a cyanobacterial mat inhabiting a sulfide-rich hot spring. Cells of both strains are short Gram-negative rods, motile with one polar flagellum (strain K–119^T) or non-motile (strain U–8^T). Both strains are obligate anaerobes, have temperature optima of 54–55 °C and pH optima of 6.9-7.2. Molecular hydrogen, acetate, fumarate, malate, pyruvate, lactate and long-chain saturated fatty acids served as growth substrates for both species; strain U-8^Twas also able to grow on propionate. All substrates were oxidized completely, H₂S and CO₂ being the only metabolic products. Elemental sulfur was obligately required for growth of strain K–119^T, whereas strain U-8^Twas able to grow also with thiosulfate as electron acceptor and on pyruvate without an external electron acceptor. The DNA G+C contents of strains K–119^Tand U–8^Twere 31.6 and 32.2 mol%, respectively. Phenotypic features and the results of 16S rRNA sequencing indicate the affiliation of the new isolates to the genus Desulfurella. The DNA-DNA hybridization with Desulfurella acetivorans was 40% for strain K–119^Tand 55% for strain U-8^T; the DNA-DNA hybridization between the new isolates was 32%. Based on the results of morphological, physiological and phylogenetic studies the following two new species are proposed: Desulfurella kamchatkensis sp. nov. with the type strain K–119^T(= DSM 10409^T) and Desulfurella propionica sp. nov. with the type strain U–8^T(=DSM 10410^T).

Key Words: Desulfurella kamchatkensis sp. nov. • Desulfurella propionica sp. nov. • sulfur-respiring bacteria • thermophilic bacteria

The EMBLaccession numbers for the 16S rDNA sequences of D. multipotens. strain K–119^Tand strain U–8^Tare Y16943, Y16941 and Y16942, respectively.

This article has been cited by other articles:

				J. R. Hall, K. R. Mitchell, O. Jackson-Weaver, A. S. Kooser, B. R. Cron, L. J. Crossey, and C. D. Takacs-Vesbach Molecular Characterization of the Diversity and Distribution of a Thermal Spring Microbial Community by Using rRNA and Metabolic Genes Appl. Envir. Microbiol., August 1, 2008; 74(15): 4910 - 4922. [Abstract] [Full Text] [PDF]