HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lien, T. Articles by Gjerdevik, K. Search for Related Content PubMed Articles by Lien, T. Articles by Gjerdevik, K. Agricola Articles by Lien, T. Articles by Gjerdevik, K.

Desulfobulbus rhabdoformis sp. nov., a sulfate reducer from a water-oil separation system

Department of Microbiology, University of Bergen, IM, Jahnebakken 5, N-5020 Bergen, Norway

Author for correspondence: Torleiv Lien. Tel: +47 555 82669. Fax: +47 555 89671. e-mail: torleiv.lien{at}im.uib.no

ABSTRACT

A mesophilic, Gram-negative, rod-shaped, marine, propionate-oxidizing sulfate reducer (strain M16^T) was isolated from a water-oil separation system on a North Sea oil platform. The optimum conditions for growth were 31 °C, pH 6.8-7.2 and 1.5-2.0 %(w/v) NaCI and 0.1-0.3% (w/v) MgCl₂6H₂O in the medium. The growth yield with sulfate was 4.6 g cell biomass (mol propionate oxidized)^-1. Strain M16^Tis nutritionally related to members of the genus Desulfobulbus, but differs in that it has no vitamin requirement and is able to utilize fumarate and malate as carbon and energy sources. Hydrogenase activity measured as hydrogen uptake was mainly membrane-bound and varied with the growth substrate. Highest activity [28 µmol min^-1(mg protein)^-1] was found in cells grown with hydrogen and lowest [50 nmol min^-1(mg protein)^-1] in cells grown with propionate as electron donors for sulfate reduction. Desulforubidin, menaquinone-5(H₂) and cytochromes of the c- and b-type were present. The fatty acid pattern was similar to that found for Desulfobulbus propionicus. The DNA base composition was 50.6 mol% G+C. Strain M16^Tis equidistantly related to D. propionicus and Desulfobulbus elongatus with 96.1 % 16S rDNA similarity. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain M16^T(= DSM 8777^T) is proposed as the type strain of a new species, Desulfobulbus rhabdoformis.

Key Words: Desulfobulbus rhabdoformis sp. nov. • sulfate reducer • 16S rRNA gene sequence • oilfield

The GenBank accession number for the 16S rDNA sequence of M16^Tis U12253.

This article has been cited by other articles:

				D. Suzuki, A. Ueki, A. Amaishi, and K. Ueki Desulfobulbus japonicus sp. nov., a novel Gram-negative propionate-oxidizing, sulfate-reducing bacterium isolated from an estuarine sediment in Japan Int J Syst Evol Microbiol, April 1, 2007; 57(4): 849 - 855. [Abstract] [Full Text] [PDF]

				S. A. Dar, L. Yao, U. van Dongen, J. G. Kuenen, and G. Muyzer Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers Appl. Envir. Microbiol., January 1, 2007; 73(2): 594 - 604. [Abstract] [Full Text] [PDF]