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1Department of Microbiology, Gifu University School of Medicine, 40 Tsukasamachi, Gifu 500, Japan
2Gifu Prefecture Health and Environment Research Center, Noishiki 4–6–3, Gifu 500, Japan
Author for correspondence: Takayuki Ezaki. Tel: +81 58 267 2240. Fax: +81 58 267 0156. e-mail: tezaki{at}cc.gifu-u.ac.jp
ABSTRACT
Three strains of creatinine-hydrolysing bacteria isolated from human urine were characterized taxonomically. They were aerobic, non-spore-forming, Gram-positive rods with the peptidoglycan of the cell wall containing lysine. MK-8 and MK-9 were found to be the major types of menaquinone. The G+C content of the DNA was 66–67 mol%. The 16S rRNA sequence of one strain (GIFU 12498) was determined and aligned with other high-G+C-content Grampositive rods from different genera. Following phylogenetic analysis, this strain was placed in the genus Arthrobacter. Arthrobacter protophormiae was the most closely related species in the phylogenetic tree, and this species also showed the highest sequence homology value (97%) with GIFU 12498. However, DNA-DNA hybridization indicated that GIFU 12498 did not belong to A. protophormiae (33·8±13·5% chromosomal similarity). The three urine strains belonged to one species because they shared more than 95% DNA-DNA similarity. It is proposed that these strains are placed in the genus Arthrobacter as a new species, Arthrobacter creatinolyticus sp. nov. The type strain of A. creatinolyticus is GIFU 12498, which has been deposited in the Japan Collection of Microorganisms (JCM) with the accession number JCM 10102.
Key Words: Arthrobacter-creatinolyticus • creatinine • rod-coccus cycle • 16S rRNA • DNA-DNA hybridization
The DDBJ accession number of the nucleotide sequence of strain GIFU 12498 reported in this paper is D88211.
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