HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sasaki, J. Articles by Suzuki, K.-i. Search for Related Content PubMed Articles by Sasaki, J. Articles by Suzuki, K.-i. Agricola Articles by Sasaki, J. Articles by Suzuki, K.-i.

Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov.

¹Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-0198, Japan
²Division of Biomolecular Characterization,The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-0198, Japan

Author for correspondence: Ken-ichiro Suzuki. Tel: + 81 48 467 9559. Fax: +81 48 462 4617. e-mail: ksuzuki{at}ulmus.riken.go.jp

ABSTRACT

An HPLC procedure which separates D-and L-amino acid isomers was applied to an analysis of peptidoglycan of 2,4-diaminobutyric acid (DAB)-containing actinomycetes. The cell wall peptidoglycans of species of the genera Agromyces, Clavibacter and Rathayibacter contain DAB and have been differentiated principally by their menaquinone profile. These peptidoglycans are known to be identical in structure, all being of the B2 type, possessing both D-and L-DAB. The type strains of all the subspecies of Clavibacter michiganensis have D-and L-DAB in almost equal proportions in their cell wall peptidoglycan as previously reported. In contrast, the type strains of Clavibacter toxicus and all valid species of the genera Agromyces and Rathayibacter contain the L-isomer of DAB almost exclusively. This characteristic is in good agreement with phylogenetic analyses based on 16S rDNA sequences and menaquinone profiles. On the basis of these data, the transfer of Clavibacter toxicus to the genus Rathayibacter as Rathayibacter toxicus comb. nov. is proposed. The isomer profile of DAB is shown to be a good taxonomic marker to differentiate these genera.

Key Words: Rathayibacter toxicus comb. nov. • 2,4-diaminobutyric acid isomers • peptidoglycan • actinomycetes • Clavibacter toxicus

The DDBJ accession numbers for the sequences reported in this paper are listed in Table 1.

This article has been cited by other articles:

				A. Matsumoto, M. Yamada, S. Omura, and Y. Takahashi Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae Int J Syst Evol Microbiol, April 1, 2008; 58(4): 1019 - 1023. [Abstract] [Full Text] [PDF]

				S.-Y. Jung, S.-Y. Lee, T.-K. Oh, and J.-H. Yoon Agromyces allii sp. nov., isolated from the rhizosphere of Allium victorialis var. platyphyllum Int J Syst Evol Microbiol, March 1, 2007; 57(3): 588 - 593. [Abstract] [Full Text] [PDF]

				Y.-C. Lin and A. Yokota Plantibacter auratus sp. nov., in the family Microbacteriaceae. Int J Syst Evol Microbiol, October 1, 2006; 56(Pt 10): 2337 - 2339. [Abstract] [Full Text] [PDF]

				R. Rivas, M. E. Trujillo, P. F. Mateos, E. Martinez-Molina, and E. Velazquez Agromyces ulmi sp. nov., a xylanolytic bacterium isolated from Ulmus nigra in Spain Int J Syst Evol Microbiol, November 1, 2004; 54(6): 1987 - 1990. [Abstract] [Full Text] [PDF]

				Y.-C. Lin, K. Uemori, D. A. de Briel, V. Arunpairojana, and A. Yokota Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae Int J Syst Evol Microbiol, September 1, 2004; 54(5): 1669 - 1676. [Abstract] [Full Text] [PDF]

				C. M. Manaia, B. Nogales, N. Weiss, and O. C. Nunes Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of 'Brevibacterium helvolum' DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. Int J Syst Evol Microbiol, May 1, 2004; 54(3): 783 - 789. [Abstract] [Full Text] [PDF]

				P. Schumann, U. Behrendt, A. Ulrich, and K.-i. Suzuki Reclassification of Subtercola pratensis Behrendt et al. 2002 as Agreia pratensis comb. nov. Int J Syst Evol Microbiol, November 1, 2003; 53(6): 2041 - 2044. [Abstract] [Full Text] [PDF]

				L. V. Dorofeeva, V. I. Krausova, L. I. Evtushenko, and J. M. Tiedje Agromyces albus sp. nov., isolated from a plant (Androsace sp.) Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1435 - 1438. [Abstract] [Full Text] [PDF]