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1Department of Bacteriology, National Veterinary Institute, PO Box 7073, S-750 07 Uppsala, Sweden
2Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44 Stockholm, Sweden
3Mycoplasma Section, Frederick Cancer Research and Development Center, National Institute of Allergy and Infectious Diseases, Frederick, MD 21702, USA
Author for correspondence: Karl-Erik Johansson. Tel: +46 18 67 40 00. Fax: +46 18 30 91 62. e-mail: Kaggen{at}sva.se
ABSTRACT
The nucleotide sequences of the 16S rRNA genes from the type strains of four goat mycoplasmas, Mycoplasma adleri, Mycoplasma auris, Mycoplasma cottewii and Mycoplasma yeatsii, were determined by direct solid-phase DNA sequencing. Polymorphisms were found in two of the 16S rRNA gene sequences, showing the existence of two different rRNA operons. Three polymorphisms were found in M. adleri, and one was found in M. yeatsii. The sequence information was used for the construction of phylogenetic trees. M. adleri was included in the Mycoplasma lipophilum cluster within the hominis group. M. auris was comprised in the Mycoplasma hominis cluster of the hominis group. M. cottewii and M. yeatsii were found to be very closely related with only four nucleotide differences, and they grouped with Mycoplasma putrefaciens in the Mycoplasma mycoides cluster within the spiroplasma group. Sequencing of two field isolates of M. cottewii and M. yeatsii, geographically distant from the type strains, showed that the 16S rRNA gene from the field isolate of M. cottewii was identical to the one from the type strain. The field isolate of M. yeatsii had only two nucleotide differences to the type strain and these were present in only one of the two rRNA operons. Sequencing of the 16S rRNA genes from two unidentified mycoplasma isolates from Nepal indicated that they should both be regarded as M. auris strains.
Key Words: goat mycoplasmas • Mycoplasma adleri • Mycoplasma auris • Mycoplasma cottewii • Mycoplasma yeatsii
The GenBank accession numbers for the sequences reported in this paper are U67943-U67946.
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