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Int J Syst Bacteriol 48 (1998), 141-149; DOI 10.1099/00207713-48-1-141
© 1998 Society for General Microbiology
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Two new Rahnella genomospecies that cannot be phenotypically differentiated from Rahnella aquatilis

Don J. Brenner1, Hans E. Müller2,{dagger}, Arnold G. Steigerwalt1, Anne M. Whitney1, Caroline M. O'Hara3 and Peter Kämpfer4

1Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
3Nosocomial Pathogens Laboratory Branch, Hospital Infections Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
2Staatliches Medizinaluntersuchungsamt, D-38124 Braunschweig, Germany
4Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität, D-35390 Giessen, Germany

Author for correspondence: Don J. Brenner. Tel: + 1 404 639 2841. Fax: + 1 404 639 4421. e-mail: djb3{at}cdc.gov

ABSTRACT

Fifty-one Rahnella aquatilis and R. aquatilis-like strains from water, snails and human sources were characterized by routine biochemical tests, carbon source utilization tests, DNA relatedness (hydroxyapatite method) and 16S rRNA sequencing. The results of the genetic methods indicated that the strains comprised three closely related species within the genus Rahnella. It was not possible to differentiate R. aquatilis from the two newly recognized species. The new species were therefore given the vernacular names Rahnella genomospecies 2 and Rahnella genomospecies 3.


Key Words: Rahnella aquatilisRahnella aquatilis-like strains • Rahnella genomospecies • taxonomy • DNA relatedness

The GenBank accession numbers for the sequences reported in this paper are U88434-U88439, U90757 and U90758.

{dagger} Present address: Alter Rautheimer Weg 16, D-38126 Braunschweig, Germany.




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