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Laboratoire de Microbiologie, Université de Neuchâtel. CH-2007 Neuchâtel, Switzerland
* Corresponding author. Mailing address:Laboratoire de Microbiologie, Université de Neuchâtel, Rue Emile-Argand 11, CH-2007 NeuchâCtel, Switzerland. Phone: 41-32-718.22.60. Fax: 41-32-718.22.31. E-mail: Michel.Blanc{at}bota.unine.ch.
ABSTRACT
The restriction enzyme profiles of 16S ribosomal DNAs (rDNAs) amplified by PCR from thermophilic heterotrophic bacterial strains isolated from composts were compared with those of reference strains. This allowed us to assign all but 1 of 16 strains to four different Bacillus species (namely, Bacillus stearothermophilus, Bacillus pallidus, Bacillus thermoglucosidasius, and "Bacillus thermodenitrificans"). This study showed that PCR restriction analysis of 16S rDNA contributes to rapid and reliable identification of newly isolated strains belonging to recognized species.
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