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Int J Syst Bacteriol 47 (1997), 1140-1144; DOI 10.1099/00207713-47-4-1140
© 1997 Society for General Microbiology
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In Vitro Culture and Phylogenetic Analysis of "Candidatus Arsenophonus triatominarum," an Intracellular Bacterium from the Triatomine Bug, Triatoma infestans

Václav Hypsa1 and Colin Dale2,*

1Institute of Parasitology, Academy of Sciences of the Czech Republic, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic
2Tsetse Research Group, Division of Molecular Genetics. University of Glasgow, Glasgow G11 6NU, United Kingdom

* Corresponding author. Mailing address:Tsetse Research Group, Division of Molecular Genetics, Institute of Biomedical and Life Sciences, University of Glasgow, Anderson College. 56 Dumbarton Rd., Glasgow G11 6NU, United Kingdom. E-mail: cd28y{at}udcf.gla.ac.uk.

ABSTRACT

An intracellular symbiotic bacterium was isolated from the hemolymph of Triatoma infestans and cultured in an Aedes albopictus cell line. 16S ribosomal DNA sequence analysis revealed that the bacterium was a member of the {gamma}-3 subgroup of the class Proteobacteria, having 96.2% sequence identity with the most closely related bacterium, Arsenophonus nasoniae, the causative agent of the son-killer trait in the parasitoid wasp Nasonia vitripennis. These bacteria share morphological features and a common tissue distribution and transmission mode. The A. nasoniae-T, infestans symbiont branch represents a lineage of insect symbionts which may be capable of horizontal transmission between phylogenetically distant host insects. We propose that the intracellular symbiont from T. infestans be classified as "Candidatus Arsenophonus triatominarum." The bacterium found in the hemocytes of T. infestans is designated the type strain of this species.




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