HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by French, F. E. Articles by Williamson, D. L. Search for Related Content PubMed PubMed Citation Articles by French, F. E. Articles by Williamson, D. L. Agricola Articles by French, F. E. Articles by Williamson, D. L.

Spiroplasma lineolae sp. nov., from the Horsefly Tabanus lineola (Diptera: Tabanidae)

Frank E. French^1,*, Robert F. Whitcomb², Joseph G. Tully¹, Patricia Carle⁴, Joseph M. Bové¹, Roberta B. Henegar², Jean R. Adams², Gail E. Gasparich^5, and David L. Williamson⁶

¹Department of Biology and Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, Georgia 30460
²Vegetable Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705
⁵Insect Biocontrol Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705
³Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick Cancer Research Facility, Frederick, Maryland 21702
⁴Laboratoire de Biologie Cellulaire et Moléculaire, Institut Nationale de Recherche Agronomique, 33833 Villenave d'Ornon France
⁶Department of Anatomical Sciences, State University of New York, Stony Brook, New York 11794

^* Corresponding author. Mailing address:Department of Biology, Georgia Southern University, Statesboro, GA 30460. Phone: (912) 681-5593. Fax: (912) 681-0845. E-mail: french{at}gsvms2.cc.gasou.edu.

ABSTRACT

Spiroplasma strain TALS-2^T from the viscera of the striped horsefly, Tabanus lineola, collected in Georgia was serologically distinct from other Spiroplasma species, groups, putative groups, and subgroups. Light and electron microscopy of cells of strain TALS-2^T revealed helical motile cells surrounded only by a single cytoplasmic membrane. The organism grew in M1D and SP-4 liquid media. Growth also occurred in 1% serum fraction medium and in conventional horse serum medium. Growth in liquid media was serum dependent. The strain passed through 220-nm filter pores, but was retained in filters with 100-nm pores. The optimum temperature for growth was 30°C. Multiplication occurred at temperatures from 20 to 37°C, with a doubling time at the optimum temperature of 5.6 h in M1D broth. Strain TALS-2^T catabolized glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine content of the DNA was 25 ± 1 mol%. The genome size was 1,390 kbp. Six isolates serologically similar to strain TALS-2^T were obtained from the same host in coastal Georgia. Three strains closely related to strain TALS-2^T were isolated from the horsefly Poeciloderas quadripunctatus in Costa Rica. Strain TALS-2^T (= ATCC 51749), a representative of group XXVII, is designated the type strain of a new species, Spiroplasma lineolae (Mollicutes: Entomoplasmatales).