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Characterization of Bordetella Strains and Related Bacteria by Amplified Ribosomal DNA Restriction Analysis and Randomly and Repetitive Element-Primed PCR

¹Laboratorium voor Microbiologie, University of Ghent, Ghent
²Department of Medical Microbiology', University Hospital UIA, Antwerp, Belgium

^* Corresponding author. Mailing address: Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: (32)9 264 51 13. Fax: (32)9 264 50 92. E-mail: Peter.Vandamme{at}rug.ac.be.

ABSTRACT

Amplified ribosomal DNA restriction analysis (ARDRA) in which the almost complete 16S rRNA gene was used as the target of the PCR assay and randomly and repetitive element-primed PCR were used to characterize 67 strains belonging to the family Alcaligenaceae. Particular emphasis was placed on strains of Bordetella pertussis (13 strains), Bordetella parapertussis (10 strains), and Bordetella bronchiseptica (10 strains) (these organisms were referred to as the B. bronchiseptica group), as well as Bordetella avium (19 strains). Our data indicate that strains belonging to the B. bronchiseptica group behave as a single species comparable to strains of B. avium. ARDRA allowed us to differentiate among all other species as well but was not useful for infraspecific typing. Randomly and repetitive element-primed PCR could be used to distinguish several infraspecific types within B. avium and the B. bronchiseptica group and allowed us to differentiate these two taxa.