HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tarlera, S. Articles by Stams, A. J. M. Search for Related Content PubMed Articles by Tarlera, S. Articles by Stams, A. J. M. Agricola Articles by Tarlera, S. Articles by Stams, A. J. M.

Caloramator proteoclasticus sp. nov., a New Moderately Thermophilic Anaerobic Proteolytic Bacterium

¹Departamento de Microbiología, Facultad de Química y Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay, The Netherlands
²Department of Microbiology, Wageningen Agricultural University, 6703 CT Wageningen, The Netherlands

^* Corresponding author. Mailing address: Cátedra de Microbiología, Facultad de Química, Casilla de Correo 1157, Montevideo, Uruguay. Phone: 5982 94 42 09. Fax: 5982 94 19 06. E-mail: starlera{at}bilbo.edu.uy.

ABSTRACT

A new moderately thermophilic proteolytic anaerobe, strain U^T, was isolated from mesophilic granular methanogenic sludge. The cells were spore-forming, motile rods that were 0.4 µ.m wide and 2.4 to 4 µ.m long and stained gram negative. Electron micrographs of thin sections revealed the presence of an atypical gram-positive cell wall. Optimum growth occurred at 55°C and at pH values between 7.0 and 7.5, with a doubling time of 30 min. The DNA base ratio of guanine plus cytosine was 31 mol%. The bacterium fermented proteins mainly to acetate, hydrogen, formate, and branched-chain fatty acids. Several amino acids, including glutamate, aspartate, arginine, histidine, threonine, methionine, and branched-chain amino acids, were also utilized. Glutamate was degraded to acetate, formate, hydrogen, and alanine. In addition, the strain degraded carbohydrates, including glucose, fructose, mannose, cellobiose, and starch, to acetate, ethanol, formate, lactate, and hydrogen. The results of a 16S rRNA sequence analysis phylogenetically placed strain U^Tin the low-guanine-plus-cytosine-content subgroup of the gram-positive phylum. We propose to classify the described strain in the genus Caloramator as a new species, Caloramator proteoclasticus. The type strain of C. proteoclasticus, strain U, has been deposited in the Deutsche Sammlung von Mikroorganismen as strain DSM 10124.

This article has been cited by other articles:

				R. N. Ledbetter, S. A. Connon, A. L. Neal, A. Dohnalkova, and T. S. Magnuson Biogenic Mineral Production by a Novel Arsenic-Metabolizing Thermophilic Bacterium from the Alvord Basin, Oregon Appl. Envir. Microbiol., September 15, 2007; 73(18): 5928 - 5936. [Abstract] [Full Text] [PDF]

				S. Tarlera and E. B. M. Denner Sterolibacterium denitrificans gen. nov., sp. nov., a novel cholesterol-oxidizing, denitrifying member of the {beta}-Proteobacteria Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1085 - 1091. [Abstract] [Full Text] [PDF]