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Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840
* Corresponding author. Mailing address: Rocky Mountain Laboratories NIAID-NIH, 903 S. Fourth St., Hamilton, MT 59840. Phone: (406)363-9376. Fax: (406) 363-9204. E-mail: markniebylski{at}nih.gov.
ABSTRACT
Rickettsia peacockii, a new species of spotted fever group rickettsiae, was identified from Rocky Mountain wood ticks (Dermacentor andersoni) collected in the Sapphire Mountain Range on the eastern side of Bitterroot Valley, Montana. DNA from R. peacockii SkalkahoT (T = type strain) in naturally infected tick tissue was amplified by a PCR assay with primer sets derived from eubacterial 16S ribosomal DNA (rDNA), rickettsial citrate synthase, and 190-kDa surface antigen (rOmpA) genes. Partial 16S rDNA and rOmpA gene sequences exhibited levels of similarity of 99.7 and 93.2%, respectively, with the sequences of the spotted fever agent Rickettsia rickettsii R. By using Giménez staining, fluorescent antibody tests, a PCR assay, and a restriction fragment length polymorphism analysis, 76 of 115 female ticks (minimal field infection rate, 66.1%) collected between 1992 and 1995 were found to be infected. The organism is passed transstadially and transovarially (minimal vertical transmission rate, 73.3%), and infections are localized in ovarial tissues. Attempts to cultivate R. peacockii were unsuccessful.
This paper is dedicated to Mort G. Peacock in recognition of his lifelong contributions to rickettsiology, inimitable cell culture talents, and help provided to us and many others in studying rickettsiae.
Present address: Division of Zoonotic Diseases, Centers for Disease Control and Prevention, Ft. Collins, CO 80522.
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